THEORIES AND EXPERIMENTS IN ANTISEPSIS. 
555 
antiseptic was then dusted into the gash and around its edges 
and developments awaited. 
For the liquid antiseptics, a certain amount of the wound 
culture was inoculated into a tube of bouillon and a definite 
amount of the antiseptic added to the tube, a control tube being 
used with every set of experiments. 
With the dry experiments two grains (0.13 gram) of the 
antiseptic were used, and every precaution taken against 
contamination or mixing of the powders. The glass scale pan 
was covered with strong alcohol and then ignited, the burning 
of the alcohol serving to still farther sterilize as well as to dry 
the pan. The powder was then removed from the container 
with a sterilized scalpel and dusted over the infected medium. 
With the liquid antiseptics a one cubic centimeter pipette 
was used. The pipette was of such a size that one drop from it 
was found to equal i-25th of a cubic centimeter. The tubes of 
bouillon were inoculated with one drop or* 1-25 cc. of the wound 
culture. The pipette was then sterilized by washing it out in 
strong alcohol and then holding it over the flame until thor¬ 
oughly^ sterile ; then 1-2 cc of the antiseptic was placed in the 
inoculated tubes. 
The first series of experiments was performed during July 
and August 1897. The material was obtained from a horse 
which had been operated upon for roaring. Tracheotomy had 
been performed and there were evidences of “ Inhalation Pneu¬ 
monia.” A small amount of pus was removed from the orifice 
made in the trachea. 
In a cover-glass preparation of the pus, micrococci were the 
only organisms discernible. Some of the pus was put into a 
tube of bouillon, and from this tube the other tubes and plates, 
used in the experiments, were inoculated. A strong putrefac¬ 
tive odor was noted in the original tube on the fourth day. 
Plate cultures were also made from the pus, and cover-glass 
preparations stained with methylen blue and carbol fuchsin, 
showed micrococci and short and long bacilli. The tube and 
plate cultures showed a vigorous growth of the organisms. 
