556 
PIERRE A. FISH. 
Ill order to determine whether in this particular case, the 
effects might be due to the products of the bacteria, or to the 
organisms themselves, or both, one of the bouillon cultures was 
filtered through a porcelain tube in order to remove the organ¬ 
isms. On July 27, 1897, eight minims of the filtrate were 
subcutaneously injected into a rabbit, with a sterilized hypoder¬ 
mic syringe. The animal was carefully observed for a number 
of days but no injurious effects were detected. On July 31, 
another rabbit received a subcutaneous injection of the unfiltered 
pus culture. A slight swelling was noticed at the point of in¬ 
oculation the next day. There was no increase for a day or 
two, but seven days after the inoculation, the swelling had in¬ 
creased considerably, until on the tenth day the swelled area 
was approximately an inch and a quarter long by three-fourths 
of an inch wide. The animal seemed healthy in all other re¬ 
spects and the swelling gradually disappeared. 
The solubility of the dry antiseptic, it would seem, must exert 
some importance with regard to its efficiency. If insoluble and 
not decomposed by the heat of the body into different com¬ 
pounds, the antiseptic has, probably, no greater action than a 
dessicating or protecting layer to the wound'. A soluble anti¬ 
septic would seem to have a greater relative efficiency, because 
its action may be two-fold. It may act directly upon the 
organisms and, at the same time, upon the somatic tissues. Its 
absorption and stimulation of the parts may thus aid in the pro¬ 
cess of repair. 
Among the preparations experimented with are the follow¬ 
ing : Dry preparations—Acetanilid, Aristol, Glutol, Iodoform, 
Salol, Thioform, and Xeroform. 
Fluid preparations—Carbolic acid, Creolin, Formalin, Hy¬ 
drogen dioxide, Dysol, Mercuric chloride, Pyoktanin, Trikresol. 
Dry Preparations .—Acetanilid.—This agent is soluble in 
water to the extent of 1 part in 194. Two grains of this sub¬ 
stance were used in a tube of bouillon with 1/25 cc. of the pus 
culture, July 31, 1897. No growth on August 1, although the 
control tube showed growth. On Aug. 2, a slight cloudiness 
