THEORIES AND EXPERIMENTS IN ANTISEPSIS. 
557 
appeared in the acetanilid tube. The cloudiness did not in¬ 
crease although observed until Aug. 5. A gelatin plate culture 
prepared as previously described, showed no growth for several 
days. 
Aristol.—This substance is soluble in ether, chloroform, oils 
and slowly in alcohol and is insoluble in water. In the test- 
tube experiment, the growth was not checked. In the plate 
experiment growth was apparent within twelve hours and kept 
increasing. 
Glutol.—This substance is composed of gelatin impregnated 
with formalin. The theory is that when applied to a wound 
the warmth of the body softens the gelatin and liberates the 
formalin, which may thus come in contact with or even pene¬ 
trate the tissues of the wound, rendering them antiseptic. In 
the test-tube experiment a very noticeable growth of the germs 
was apparent upon the day following the inoculation of the 
tube. In the plate experiments two sets of plates were used } 
one set of agar and one of gelatin. The agar plates were placed 
in the incubator, and growth was restrained by the glutol for 
about 48 hours. The gelatin plate showed no signs of growth 
until the second day after inoculation. 
Iodoform.—A small amount of this agent was taken from the 
stock-bottle and placed in a tube of bouillon to test its own pur¬ 
ity from germs. There was no growth after two days. The 
tube experiment in this case, differs from the others, in that a 
larger proportion of the culture was introduced into the tube, as 
follows: i/5th cc. of the pus culture was used and one grain of 
iodoform. In two tubes inoculated with these proportions, 
growth occurred within fifteen hours. In the plate cultures, 
the gelatin liquefied on the day following the inoculation but 
there did not appear to be growth in colonies as in the other 
plates. The gelatin remained quite clear but liquid. 
Rideal (4)—in discussing iodoform states that the power of 
iodoform has recently been much disputed. While the Lancet 
asserts that it is a much better antiseptic than most other sub¬ 
stances which are used for the same purpose, and is of much 
