THEORIES AND EXPERIMENTS IN ANTISEPSIS. 
559 
Fluid Preparations .—Carbolic acid.—This agent was used 
in 2.5 per cent, and 5 per cent, solutions. 1-25 cc. of pus culture, 
and one cubic centimeter of the antiseptic being inoculated into 
the tubes. After twenty-four hours there appeared to be a slight 
turbidity in the 2.5 per cent, tubes, but after nine days there 
was no appearance of gain. In the 5 per cent, tubes there was 
no growth whatever. The experiments were repeated in July, 
1898, with a 2 per cent, solution. A very slight turbidity 
appeared, but did not extend. 
Creolin.—This agent was used in a one per cent, solution. 
The proportions of the pus culture and antiseptic were the same 
throughout all of the experiments. The addition of the creolin 
to the tube caused an opacity, which rendered it difficult to dis¬ 
tinguish any growth. In such cases the tubes were put in the 
incubator over night, and a loop from these tubes was intro¬ 
duced into a fresh tubeif growth had occurred in the former 
it would, in due time, appear in the latter, and there would be 
no opacity to obscure detection of the new growth. Fresh tubes 
inoculated from the creolin tubes showed growth upon the fol- 
Organism. 
Proportion of 
Formaldehyde 
inhibiting 
growth. 
Proportion 
allowing 
some growth. 
Remarks. 
Growth poor— 
Staphylococcus 
1-5000 
I-IOOOO 
1-10000, and much 
pyogenes aureus. 
delayed 1-20000. 
Bacillus typhosus. . 
Bacillus coli 
1-15000 
1-20000 
Very scanty growth. 
After 72 hours in- 
communis. 
1-7000 
I-IOOOO 
cubation. 
Bacillus anthracis.. 
1-15000 
1-20000 
Scanty growth on 
6th day. 
Spirillum cholerge.. 
1-20000 
Bacillus mallei . . 
1-20000 
... 
Bacillus pyocaneus. 
1-7000 
I-IOOOO 
On the 3d day. 
Bacillus lacticus . . 
Bacillus butyricus 
1-20000 
. . . 
(Hueppe). 
1-20000 
... 
Micrococcus 
prodigiosus. 
1-20000 
• • . 
