12 
The Colorado Experiment Station 
stained with aqueous fuchsin, the organism is found to be a 
short rod with rounded ends, for the most part single, more 
rarely in short chains of four to six elements, and occasionally 
in long filaments. Many of the rods appear slightly curved and 
wedge shaped; the rods taken directly from diseased stems are 
usually shorter, and when stained with aqueous fuchsin, appear 
to take the stain more deeply at the poles. 
The individual rods usually measure about 1.2 to 2.4^ x .5 to 
.S/x; the majority are about 2.1/* x .7/*; the filaments vary in 
length from 20.2/* to 37.2/*. 
2. Sporangia. 
No sporangia have been observed. 
3. Endospores. 
No endospores have been observed in agar cultures four months 
old. 
4. Flagella. 
When stained by Loeffler’s (1) method, it was possible to demon¬ 
strate from 1 to 4 flagella attached to each pole. Following 
Migula’s classification, this bi-polar attachment of the flagella 
places the organism in the genus Pseudomonas. A 24 hour agar 
(1) Centralbl. f. Bakt., 1889, 6, p. 209; 1890, 7, p. 625. 
culture, examined in the hanging drop, exhibits very actively 
motile rods with rounded ends, from two to four times as long 
as broad, and which occur, for the most part, singly and occa¬ 
sionally in short chains and filaments. 
5. Capsules. 
No capsules have been observed when stained by Welch’s 
method. (2) 
6. Zoogloea. 
No zoogloea have been observed. 
7. Involution Forms. 
Films made from an agar culture four months old and stained 
with carbol fuchsin show many degenerative forms. Long irreg¬ 
ular rods which stain unevenly, giving a granular appearance, 
and usually darker at the poles, are among the most common. 
No unusual or strikingly characteristic forms have been observed. 
8. Staining Reactions. 
The organism stains readily with the ordinary aqueous stains, 
anilin gentian violet, and carbol fuchsin. 
Gram’s Stain. 
The results from repeated efforts at staining by Gram’s method 
were not sufficiently sharp and decisive to warrant any positive 
conclusions; the stain was neither entirely lost nor was it re¬ 
tained in its original depth and brilliancy. Both the anilin 
gentian violet and the iodine solution were used for one and 
one half minutes and the preparations were left in absolute 
alcohol for five minutes. When examined, the germs were dis¬ 
tinctly stained, but not as intensely as those which had not been 
treated with the alcohol and for this reason, alone, the organism 
is here reported as Gram negative, while in reality it is neither 
positive nor negative. 
Loeffler’s Methylene Blue. 
Stained with Loeffler’s methylene blue, the protoplasm of the 
organism appears very coarsely granular, so much so, in fact, 
as to give the rods almost a striated appearance. 
Neisser’s Stain. 
Neisser’s stain brings out a more or less granular structure to 
(2) Bull. Johns Hopkins Hosp., 1892, 3, p. 128. 
