— i6 — 
cally like the loco and got the same reactions with the re¬ 
agents above described. I tried treating with sulphuric acid 
first, and afterwards I tried hydrochloric, then tartaric, then 
acetic acids. When the sulphuric acid extract was evapor¬ 
ated down, it gave a blacker residue than the other acids. 
Nearly all the samples when treated with alcohol, there sep¬ 
arated out crystals of lime, that were insoluble in the alco¬ 
hol. At Dr. Wiley’s suggestion I obtained a copy of the 
American Chemical Journal , Vol. II., No. 8, and carefully fol¬ 
lowed out the method recommended there, with the same re¬ 
sults as before. Prof. Sayre visited me in 1890 and called 
my attention to what Prof. Power had done, and almost at the 
same time I saw the article of Profs. Power and Gambier in 
the Pharmaceutische Rundschau and in the Rocky Moimtain 
Druggist. This year I secured other specimens and carefully 
followed the method there laid down. The results were the 
same as in former years. Also tests were made with rabbits 
by feeding a teaspoonful of the aqueous extract every hour, 
from 8 to 5 p. m., on P'riday, Sept. 2, 1892 to Monday, Sept. 
5, 1892, with no bad effects. During the summers of 1891 
and 1892, considerable time was spent on the analyses of 
the loco plant. I visited Livermore or vicinity five times, and 
made three post mortems. A brief outline of the post mortems 
is here appended. The first post mortem was made on a 
3-year-old colt, the property of Mr. C. The colt was brought 
in from the range, and was in very poor condition. 
When driven around the yard he had the peculiar high 
step so often described as being a characteristic symptom of 
loco. He was roped, thrown, and his throat cut. The post 
mortem appearance was as follows : The heart, lungs and. 
liver were normal in appearance. The stomach was com¬ 
pletely covered with bots, and contained, besides, a large 
number of thread worms. The intestine connected with the 
stomach (duodenum) was filled with sand. I estimated that 
about two gallons were in the intestines. (When it is 
known that the post mortems are held from twenty to thirty 
miles from the College, and in such conditions as we can ob¬ 
tain the animals, only estimates can be made of some things).. 
The brain had a clot of blood at the base of it. I advised the 
owner (because he had twenty horses suffering from like symp¬ 
toms) to put the animals on good, green feed, so it might 
act as a physic, and carry the sand out of the system. I recom¬ 
mended a tonic of nux vomica. He told me the affected 
animals improved so they were all finally sold. The sand, as 
I think, comes from the animals not being properly salted, 
and from eating the alkali soil. 
