— i 7 — 
s eed wlndi wa s in the hay was not crushed in the grinding 
oi the sample and would yield so good as none of its fat in 
Ablrlfu i°- ur f tre u atn jent with ether. We subsequently 
established this fact by direct experiment with whole un¬ 
hulled seed. With this one exception, if it is an excep¬ 
tion, there is no clearly indicated difference in the amount 
o crude fat present at the different stages of develop- 
ment examined in this study. F 
NITROGEN FREE EXTRACT. 
The substances embraced under this name, having 
heretofore been determined by the difference between the 
sum of the proteids, crude fiber, fat, ash and moisture, and 
one hundred, will vary inversely as and quite nearly with 
the substance present in the largest quantity, which is the 
crude fiber. By this we mean that, if the crude fiber is 
high the nitrogen free extract, which includes sugar 
starch, etc., called carbohydrates, will, as a rule, be lower 
than in another sample having less crude fiber. If the di¬ 
rect determinations have been made with care, the nitro- 
gen free extiact determination will be quite accurate 
enough tor all purposes. 
Laboratory Samples. 
Nitrogen free extract in first cutting alfalfa hay:— 
1. Plants not in bloom. 
2. “ not quite in bloom. 
3* “ half bloom. 
4- “ full bloom. 
5 - “ full bloom. 
6. “ full bloom. 
7- “ full bloom. 
8. “ full bloom. 
9. “ full bloom. 
10. “ just past full bloom 
11. “ full seed. 
Average. 
Nitrogen free extract in second 
1 
2 
j 
4 
5 
6 
7 
Plants not in bloom 
coming in bloom 
half bloom. 
half bloom. 
full bloom. 
past full bloom. 
past full bloom. 
Average. 
Per cent. 
.29.79 
. 32 - 9 I 
. 32.50 
.27.85 
.37-64 
.30.59 
. ; • - 33-24 
.33-n 
. 3 i- 4 i 
.30.41 
.29.22 
. 3 I -69 
cutting alfalfa hay:— 
Per cent. 
.36-49 
.31-58 
. 33-29 
.28.90 
.32.02 
. 39-45 
.38.13 
.34-27 
t 
