, 4 Colorado Experiment Station. 
used as a basis for preparing the different agars together with the 
composition of the various solutions from which the agars were made 
is given below. The solid medium was prepared by adding 15 grams 
of agar to each 1000 c. c. of solution. 
* Water soluble salts in soil used as a basis for synthetic agars. 
Per Cent 
Ca SO* 15.902 
Mg S 0 4 - 
k 2 SO* ------ 
Na, SO* - - - " " 
Na.,CCL - - - - 
2.942 
3.387 
15.264 
4.813 
Na Cl - - - - 
Na N 0 3 
Silicic Acid 
Loss (water, organic matter, etc.) 
34.145 
22.781 
.252 
.471 
The water soluble amounted to 2.97 per cent of the air dried 
material. 
Solution lacking Calcium Sulphate (Ca SO*) 
Distilled water 
Na, SO* 
Na, C 0 3 
Na Cl 
Na N 0 3 
K, SO* 
Mg SO* 
Mannite - - 
1000.00 c. c. 
9 0668 grams 
2.8589 grams 
20.2621 grams 
13.5319 grams 
2.0118 grams 
1.7475 grams 
15.0000 grams 
Solution lacking Sodium Carbonate (Na 2 CO*) 
Distilled water 
Ca SO* 
Na, SO* 
N.a Cl 
Na NO3 - - 
K, SO* - 
Mg SO* 
Mannite 
1000.00 c. c. 
9.4457 grams 
9.0668 grams 
20.2621 grams 
13.5319 grams 
- 2.0118 grams 
- 1.7475 grams 
15.0000 grams 
Solution lacking Sodium Chloride (NaCl.) 
Distilled water ----- 1000.00 c. c. 
Ca SO - - - - - 9.4457 grams 
Na, Sol ------ 9.0668 grams 
Na'CO, - - - 2.8589 grams 
N a NO s - - - - - - - 13.5319 grams 
K, SO*" - - - - - - 2 • 0118 § rams 
Mg SO* - - - - - - - 1.. 7475 grams 
Mannite - - - 15.0000 grams 
♦Furnished by Dr. Headden. Bui. 155, p. It, Analysis XV, Colo. Kxp. Sta. 
