Bacteriological Studies oe the Fixation of Nitrogen. 
Solution lacking Sodium Nitrate 
Distilled water - 
Ca S0 4 - - 
Na, S0 4 
Na, C0 3 - - 
Na Cl - - 
K 2 so 4 - 
Mg S0 4 
Mannite - 
(Na No?,.) 
1000.00 c. c. 
9.4457 grams 
9.0668 grams 
2.8589 grams 
20.2621 grams 
- - 2.0118 grams 
1.7475 grams 
- 15.0000 grams 
Solution lacking Sodium Sulphate 
Distilled water - 
Ca S0 4 
Na. CO. - _ 
Na Cl - 
Na N0 3 - 
K 2 so 4 - 
Mg S0 4 
Mannite - 
(Na, SO,) 
1000.00 c. c. 
9.4457 grams 
2.8589 grams 
- 20.2621 grams 
13.5319 grams 
- - 2.0118 grams 
1.7475 grams 
- 15.0000 grams 
Solution lacking Magnesium Sulphate (Mg SO,) 
Distilled water - 1000.00 c. c 
Na. S0 4 
Na. C0 3 
Na Cl 
Na N0 3 
K 2 so 4 - 
Mannite - 
Solution lacking Potassium 
Distilled water 
Ca S0 4 
Na. S0 4 - 
Na. C0 3 
Na Cl - 
Na N0 3 
Mg S0 4 
Mannite - 
Solution lacking Mannite 
Distilled water 
Ca S0 4 
Na. S0 4 
Na. C0 3 
Na Cl 
Na NO. 
K 2 so 4 - 
Mg S0 4 
- - y .4457 grams 
9.0668 grams 
2.8589 grams 
20.2621 grams 
13.5319 grams 
- - 2.0118 grams 
“ 15.0000 grams 
Sulphate (K2SO,) 
1000.00 c. c. 
9.4457 grams 
9.0668 grams 
2.8589 grams 
- 20.2621 grams 
13.5319 grams 
- - 1.7475 grams 
“ 15.0000 grams 
( CeHuOe) 
1000.00 c. c. 
9.4457 grams 
9.0668 grams 
2.8589 grams 
20.2621 grams 
13.5319 grams 
- - 2.0118 grams 
1.7475 grams 
1 lie^ chemical leaction of these solutions was left unchanged, 
^ngars of four different strengths were prepared from each solution 
by diluting the solution once, twice and three times with an equal 
volume of distilled water. The resulting agars, then, contained the 
