190 
BREEDING OUT—METHODS OF FEEDING 
Pupae were collected in the morning and evening and placed in 
breeding-out tubes half filled with water and plugged with cotton. 
Each morning the newly emerged mosquitos were transferred to 
biting-jars. These biting-jars were modified from those described 
bv Stephens and Christophers. After trying pickle jars and malted 
milk jars with much impatience, jars made of lantern chimneys were 
used. I hese were covered on both ends with crinoline gauze, 
fastened with a string or a strong rubber band. Inside the jar was 
placed a circular ring platform of stiff paper, which many of the 
mosquitos used as a resting place. About twenty mosquitos would 
be placed in a jar over a small Stender dish* containing water on a 
Petri dish cover with a raisin or a piece of date for food. The jar 
would then be placed on a shelf in a dimly-lighted place, protected 
rom ants by kerosene cups. Adult mosquitos may be kept alive 
or several days in such jars if they are fed with dates or raisins 
an a few drops of water, but if fed daily they will not bite and 
SUC 3 ooc * with alacrity. Anophelines do much better if they have 
one or two preliminary blood-meals before being fed with dates, 
laisins or bananas. In several experiments it appeared that in the 
mid-gut of mosquitos gorged with stale banana and the associated 
ac eria and yeasts, the fermentative acid contents either destroyed 
age a of gametes, or in some other way prevented the 
e opment of zygotes in the mid-gut of susceptible mosquitos, 
e experiment a patient whose blood contained almost as many 
s as eucocytes was bitten by four varieties of mosquitos; 
ee of the four being susceptible species, yet none became infected. 
Ihm failure might be attributed to the feeding on bananas. 
dates ra'c‘ le ra °;r tOS WGre USec * * or biting they were fed with 
and disse T S and bananas ’ whe never they were to be kept for biting 
Tl ZTTll “ 7 s f ° Und th3t th ° Se fed “* dates and raisins 
mosaicsV factions, while the mid-guts of banana-fed 
caused the H y C ° ntamed man >- >’ easts a ” d bacilli which 
“ntation d /° me ° f the mOSCluitos ' a PP a *cntly as a result of 
' and frequently yielded partly softened, tender and 
* A • Stender-dish ' is one with a oflhe dish ,JE 
