disintegrated intestinal tracts, which were removed intact with 
difficulty, and were occasionally lost. Bananas should never be 
used as food when dates or raisins can be obtained. 
Mosquitos were kept in these biting-jars until a suitable case of 
malaria presented. The blood of patients selected to be bitten 
contained gametes or sexual forms of the malarial parasite in their 
circulating blood in numbers sufficient to infect susceptible 
anophelines. The method of determining this point will be 
considered later. 
BITING AND INFECTING EXPERIMENTS 
The earlier biting experiments were conducted at about 8 
o’clock in the evening. After selecting a patient the jar of 
mosquitos was placed on the patient’s forearm and covered with a 
heavy towel to prevent the disconcerting effect of light. Females 
several days old, that had been fed exclusively on dates or raisins, 
would generally bite greedily, and would feed on successive or 
alternate nights if given an opportunity. Most females would bite 
twenty-four or more hours after emerging, but before that period 
they would generally make no attempt to do so. When a jar is 
placed over a patient’s forearm the mosquitos that are going to bite 
will almost always do so within a few seconds. If they show no 
inclination to visit the arm, a few gentle puffs into the opposite end 
of the jar, through the crinolin gauze, will make them change their 
position and frequently take up one on the patient’s forearm. 
Another method that was used successfully was to have the patients 
bed in the dark. If the jar were then gently tapped two or three 
times and aimed at a light down the ward some distance away the 
mosquitos would always take up a position on the gauze facing the 
light. The patient’s forearm could then be carefully interposed 
and placed on the gauze, when the mosquitos would frequently take 
the hint and feed. Strong lights are powerfully attractive to 
female Anophelines and interfere considerably with successful 
bitings unless the jars are darkened by means of a thick cloth, the 
patients taken into a dark room, or the mosquitos very hungry. 
Later, it was more convenient to conduct the biting about 
