206 
Experiment No. 47.—February 19, 1909, 8 p.ra., jar containing Ce. albimana 
A. pseudofundipennis and Arr. (?) malefactor was applied to No. 53,937, who** 
receiving 30 grains of quinine daily when his blood contained one crescent to 200 
leucocytes = approximately + 30 gametes per mm.*. The mosquitos were given 
but one blood feeding and subsequently fed on raisins and dates. 
Upon dissection on February 23-5 :— 
9 A. fscudofunctifennis were not infected 
4 Arr. (?) malefactor „ „ 
2 Ce. argyro tar sis ,, 
3 Ce. all i man a „ „ while 
1 Ce. al him ana contained three zygotes, and 
1 Ce. albimana contained four zygotes. 
Experiment No. 31. January 25, 1909, a jar containing A. fseudofunch- 
pennis, <?. argyrotarsis and Ce. albimana were fed from 53343, tertian malaria 
fever, whose blood contained four gametes per ,00 leucocytes 
Upon dissection, January 30 : — 
3 - A. pseudofunctipennis were not infected; 
1 Ce. argyrotarsis was not infected ; 
1 Ce. albimana was not infected ; while 
1 Ce. albimana contained two zygotes. 
Notes on the Bionomics of some of the Anophelines 
The period of incubation of the ova of Ce. albimana , A. pseudi 
punctipennis and Arr. (?) malefactor was estimated as abou 
t nrty six hours under the laboratory conditions, the temperature 0 
t ie air and water ranging between 78° and S2 0 daily, an easier 
n o\\ exposure with direct sunlight for three or four hours in th 
morning, and diffused sunlight the remainder of the day. Th 
gt, aie aid in the geometrical patterns usually seen, and at firs 
, ■ ani ^ ' vb * te colour, becoming in a few hours quite black, wit! 
white lateral air chambers. 
t C ^ TVa ^ pCnod varies with the species, food, efficient tempera 
g , a ; d env ~ n t; for example, ova of dr, (? 
Zlt T C J' alb ~ of about the same age were exposec 
air anT ™ 6 l t0 an ident * cal environment, food, water 
Arr CP) 11 ” Ut WilCn Ce ' albiman a had pupated tlie larvae of 
Ce al him mae were on] y grown. In one experiment 
malefact ana arVaC pupated with »n twelve days, while Arr. (?) 
examinatio re ^ Ulre S1Xteen to twenty days. Subsequent 
the Tr s 1 ° ^ fr ° m malefactor pools and from 
ma raLts oi male factor larvae indicate that the latter 
