21 I 
specimens of malefactor usually contains much dark brown, 
unidentified vegetable fibres, brown organic debris, and comdia 
resembling those of Pestalozia trunculata, Leptosporum bifurcatum, 
Parainoecia, etc., Rotifer vulgaris. This indicates that Ce. albimana 
and A. pseudopunclipennis prefer sunny pools, while Arr. (?) 
malefactor prefers shady ones, where there is a relative absence of 
chorophyll-bearing forms. This is not intended as an absolute 
statement, because during the dry season, and in certain situations, 
malefactor and albimana will be found together in the same streams 
or pools, but it indicates certain different tendencies in the respective 
species. 
BLOOD FEEDING NECESSARY FOR ANOPHELINES 
A blood meal seems to be necessary for the development of the 
ova of Anophelines. In Experiment No. 40, to determine this point, 
male and females, Ce. albimana and A. pseudo punctipenms were 
placed in a breeding jar and fed on vegetable food and water 
daily, but they received no blood meals. Upon dissection of females 
as they died, none showed any development of the ovaiies. 
PARTHENOGENESIS 
If, however, there be given one blood meal the ova may develop 
even in virgins kept out of contact with males. In the latter instance 
(with Stegomyia calopus) the ova have never developed into larvae. 
Experiment.— V irgin Anophelines bred out from single isolated pupae wer 
transferred to one jar entirely out of contact with males. I here were t re 
albimana and two . 4 . pseudopunetipennis. When applied to the arm a e 
albimana drew blood; neither of the A. pseudoputieltpennis would 1 
following day one A. pscudofunctipennis died, but the remaining one, wit 
Ce. albimana bit again upon application to the arm. lhese were adde to an 
jar of virgin females, Ce. albimana and A. pseudopunetipennis, most o 
drew blood readily. Upon dissection none of the A. pseudopututiftnnis s owe a 
development of the ovaries. One Ce. albimana, about fourteen days old, containe 
ova 0-48 min. long. The single spermathecae of the mosquitos were ** a ""“* ’ 
1 .1 _ * /liwolnn into I\ dt. 
and in no instance contained spermatozoa. 1 he ova w 
in water, and upon microscopic examination were foui 
food material, but no partly developed larvae, such as 
,a would not develop into larvae 
found to contain finely granular 
would be seen in fertile ova 
of this size. 
