212 
A further experiment with Stegomyia calopus indicates that 
whereas ova may develop in size in the ovaries of unfecundated 
virgins, they are always sterile and never contain partly developed 
larvae, but granular, undifferentiated protoplasm or food material. 
A. November n a jar containing five female Stegomyia ealofus which bad 
been separated individually as pupae, and always out of contact with mate 
emerged November 9. They were applied to the’arm and three bit and drew 
blood ; they were not as voracious as Anophelines, but behaved with the caution 
and timidity characteristic of Stegomyia. After fourteen days these mosquitos bad 
not oviposited. Two were dissected and the spermathecae found to be free from 
spermatozoa, but their ova were well developed, 0-560 mm. in length, 0-184 mm. ir. 
width. 
B. Bred out nineteen virgins, Stegomyia ealofus, in the same manner, from 
isolated pupae. Upon applying to arm, sixteen out of nineteen bit and apparently 
drew blood, but there was no sensation of stinging. The next day when applied, 
only three mosquitos bit. These may have been the ones that did not bite the day 
u °j 6 Thirty-seven days after the first biting, eight of these virgins were living, 
the dead ones having become water-sprawled. Forty black ova were found in the 
water dish this morning. 
41 days after the first biting six ova were found in the dish ; 
54 days after the first feeding one dead female was found, and upon dissection 
contained no ova ; 
69 days after the first feeding a dead female was found to contain twenty 
well-developed ova, 0-560 mm. in length ; 
61 days after first feeding forty-three black ova were found in the dish, 
completely developed, 0-720 mm. in length, 0-240 mm. in width; 
!hl!fi nO1 ! e f Of . theSe L 0Va developed later into larvae. One hundred and four davs after 
the first feeding the remaining female is still living. 
EFFECT OF SALT AND SEA WATER ON ANOPHELINE LARVAE 
In general, the effect of an irritating, toxic, or otherwise unusual 
fluid on mosquito larvae is to hasten pupation. A number of exped¬ 
ients were tried with sea water, salt water and solutions of the heavy 
neta s, and in most instances in the more concentrated solutions, 
the latvae were not killed within twenty-four hours, they 
pupate and occasionally the period of pupation was shortened; 
fi° b 11 ’ r 1 instance ’ ,n a Strict sea water were used as a larvacide 
rst e feet would be to hasten pupation and thus increase the 
number of Anophelines in the district, and if later the sea water 
ecame nuted by rain, several species of malaria-transmitting 
op ie mes might breed in it without difficulty, notably Ce. albimam 
and larsimaculata. On this account sea water could not be used 
] ^ egree of success as a larvacide for Anophelines, except in 
large quantities and in certain locations. 
