2 3 2 
and three outside, with, of course, uninoculated controls in both cases. 
The uninoculated controls throve and call for no remark, except that 
one in the cold chamber died after 34 days of no obvious cause, there 
being- only a little lung congestion evident in post mortem. Of the 
inoculated, all are still alive, and those in the cold chamber have now 
been in it 137 days, and seem quite fit and well. One female has 
aborted twice, but whether from the cold, the tubercle, or other cause, 
cannot be definitely stated. Only three were apparently mature at 
the time of inoculation, and their weights in the case of two inoculated 
have risen in one case from 400 to 653 grams, and fallen in the other 
from 77 2 to 747 ; whilst the third, uninoculated, has risen from 622 to 
794. Their temperatures have not shown any very striking varia¬ 
tions ; but the swelling over the site of inoculation burst fully a month 
sooner in the two controls outside, in which rupture has occurred, than 
in the one in the cold chamber, which developed suppuration; and 
this, as far as it goes, points to some diminution of the virulence of 
the bacilli in the cold chamber. 
Four guinea-pigs have since (22 March) been inoculated with 
human tubercle, and three of them are at present under observation, 
one having died soon after inoculation. 
Six guinea-pigs were inoculated with T. gambiense on the 14^ 
December, and, as usual, placed three in the cold chamber and three 
outside as controls. All failed to show trypanosomes in the peripheral 
blood. One in the cold chamber died 16 days after inoculation, but 
no trypanosomes were found in the heart’s blood post mortem, and 
it probably died of other causes. The other five were again inoculated 
on the 4th March, and again failed to show trypanosomes. But a 
third inoculation on March 30 has been successful and interesting, 
inasmuch as, whereas the three outside showed parasites in their 
peripheral bloods on the fifth day, of the two inside one only showed 
them very scantily on the eighth day, and has not shown them since, 
the other has so far not shown them at all: a result pointing strongly 
towards at least a delayed development in those in the colder air. 
Several rats have been inoculated with cultures of pneumococcus, 
but so far no marked difference has been observable in their symptoms, 
whether in or out of the chamber, and none have died so that the} 
remain under observation, and the final result will only become 
apparent later. 
