329 
Thus choline was absent in the blood practically at the height of 
the parasite infection. 
According to Plimmer* the brains of rats that have been 
inoculated with the human trypanosomes have shown the peculiar 
perivascular infiltration. It is interesting to note from my 
observations that choline failed to enter into the blood, or m other 
words the products of nerve degeneration have not passed into the 
blood. 
IX. Effect of products of nerve degeneration in the cerebrospinal 
fluid (i.e., choline and nucleo-proteid) from the case of 
sleeping sickness—on the coagulation time of blood and the 
percentage of haemoglobin in the rat. 
Before inoculation 
of cerebrospinal 
fluid 
After inoculation of the same intraperitone 
ally 
intraperitoneally 
tst day 
3rd day 
4th day 
5 th day 
6th day 
min. sec. min. sec. 
min. sec. 
min. sec. 
min. sec. 
min. sec. 
min. sec. 
Coagulation time 
(By Wright’s 
method at half 
blood heat) 
4 25 t0 4 3 ° 
3 »5 
7 5 
6 40 
6 55 
10 46 
Haemoglobin ... 
(Sahli) 
*20% 
80% 
55 % 
4 °% 
60% 
80% 
We may not be able to draw any inferences from the smallness 
of this experiment, but one fact remains that the cerebrospinal 
fluid from the case of sleeping sickness contained a product which 
influenced the negative and the positive phase of coagulation, and 
so far as is known on the subject, it may be the nucleo-proteid. 
X. Sections of the brain tissue. 
The brain tissue was kindly lent to me by Dr. Stephens to whom 
ray thanks are due. 
The tissue was embedded in paraffin, sections were cut an 
stained with haematoxylin and eosin. 
* Allbutt and Rolleston. System of Medicine, Vol. II, I art II, p 
