46 7 
into quarters . T he films were dehaemoglobinised, fixed in absolute 
alcohol, and stained by the Romanowsky method. 
RESUME OF PREVIOUS WORK ON LATENT BODIES 
OF TRYPANOSOMES 
The various flagellate forms of 1 rypauosoma gambiense have 
been so often described by many competent workers that it is 
needless to discuss them further in detail. Suffice it to say that, 
long, thin trypanosomes may occur, especially at the beginning of 
infection in rats and guinea-pigs, and shorter, stout or stumpy 
forms later. Trypanosomes intermediate in character also are 
found. 
The so-called rounded, latent or encysted forms, which are 
non-flagellate, must be discussed in greater detail. Marked 
attention to these non-flagellate forms of trypanosome was first 
drawn by Moore and Brcinl (190/- 8 ) in mammalian trypanosomes, 
though Dutton saw rounded forms of an amphibian trypanosome 
on the Congo in 1903-5. Moore and Breinl (i 9 ° 7 )> working on 
stained material, stated that latent forms occurred in the internal 
organs, especially during the periods when the flagellates weu 
decreasing or absent from the peripheral blood of the host. These 
authors give a curve showing the variations in the numbers o 
T. gambiense in infected rats; they do not, however, give any 
numerical data in support of the graph. A few other workers 
mentioned rounded bodies in connection with trypanosomes, amor 
whom Hindle (1909) may be noted. 1 he significance of t e aten 
bodies is still a disputed point, and it was leccntly' state 1 
review that more ev idence was required to show that they constitu e 
part of a life-cycle in the vertebrate host. * . , 
In the present paper the general statements of Moore an ’ 
regarding forms of T. gambiense in the internal organs o ra ^ 
are shown to be accurate, and many details, as seen in t le 
parasite, as well as numerical data from daily counts 0 
trypanosomes, are supplied in proof of the significance o ^ 
bodies. Animals moculated with T. r/.odesiense (Stephens and 
Fantham) have also been carefully' investigated. 
: S.S. Bureau, Bulletin No. 15 (March, p- io2 - 
