•475 
In the formation of rounded, latent bodies, as seen in vitro , a 
portion of the body of the flagellate, after passage of the 
blepharoplast towards the nucleus, is thrown off, and the flagellum 
is discarded (text-fig. i)- Examination of preparations of the 
internal organs (lungs, heart, spleen) of the host shows that a 
similar method of formation of the post-flagellate stages of the 
parasite usually occurs in the internal organs (figs. 1-5). However, 
in the peripheral blood, after careful searching of a sufficient 
quantity of hlood (]- cubic millimetre), a few rounding or rounded 
parasites can generally be seen (figs. 12, 17-20). Also, in the 
internal organs, stages of the parasite intermediate between the 
flagellate and non-flagellate forms may be seen (fig. 33 )- 
The post-flagellate or latent bodies vary in size (figs. 2-16, 22 >. 
This variation is due to two causes : (ij the formation of 
non-flagellate parasites from trypanosomes of different breadths, 
and (2) the occasional division of large post-flagellate forms by 
binary fission, an example of the simplest schizogony (fig- 2 3 ,- 
This fission, so far as my researches go, is infrequent in the cast- 
of T. gambiense and T. rhodesiense. It has been observed that 
division of flagellate trypanosomes may immediately precede the 
formation of latent bodies. 
Broad forms of T. rhodesiense , with posterior nucleus, may 
form relatively large latent bodies. As the nucleus is at or near t ie 
posterior (non-flagellar) end of the parasite, there is little o 
body discarded in that region when rounding occurs. The ki ne\ 
shape of the nucleus of some specimens of T. rhodesiense. is seen 
in their latent bodies (fig. 27), and the nucleus lies to one 
the rounding body (figs. 17, 18)- ^ * s not easy, however, 
differentiate between the latent bodies of 7 . rhodesiense an 
gambiense. ,• 
Moore and Breinl describe a stainable band 01 0 ac< 
connecting the nucleus and blepharoplast of certain speci 
7 . gambiense at or near the maxima. An intei action ta■ es p 
between the blepharoplast and nucleus. Aftei this tie 
of latent bodies proceeds. During the researches now recoi' ’ 
there was no good evidence found in support of Moore ai 
views. The stainable band was seen in some stout (probably old) 
parasites, and in parasites at periods near the deat 1 
