Equal amounts of the serum and emulsion of red corpuscles 
were then mixed together and drawn up into a capillary tube which 
was placed vertically in the incubator at 37 0 C. 
The results of such experiments may be briefly summarised. 
With the exception of a few cases in which there appeared to be a 
trace of agglutination they were all negative. Similar negative 
results were obtained when the sera were examined for the presence 
of iso-agglutinins and also when the serum was replaced bv citrated 
plasma. 
At first one was somewhat at a loss to account for these 
apparently conflicting results, as even the sera of animals 
where the cover-slip preparations exhibited the most marked 
auto-agglutination of the red cells gave invariably negative results 
when examined for auto- and iso-agglutinins by this method. 
Later it was observed that when the blood from one of the 
infected animals was allowed to flow from a vein of the ear into 
.1 watch-glass containing a small amount of citrated saline solution, 
the red cells quickly sank to the bottom in little clumps, producing 
T H^* rked dCgree thc sandy a Ppearance described by Dutton and 
0 • When, however, the watch-glass and salt solution were 
nie to 37 C. and the blood dropped in as previously, this 
appearance did not result; the red cells remained suspended for 
a considerable time and only subsided gradually, as in the case of 
. 00c • S° long as the temperature of the watch-glass and 
salt solution was kept at 3 /°C., no agglutination resulted, but as 
on as the temperature was allowed to fall to about 18° C., the 
was obtained t0?et ^ er int ° tdumps an ^ the typical sandy appearance 
c e ob vat served to ind . cate rature ved an 
,hc T 2 m deVe, ° pment of the Phenomenon. Accordingly, 
of tests were eXP r me f but this occasion three sets 
the^^t^ r 7 C P,3Ced “ incubator at 3^, 
last were kept in L ? Y temperature ( l8 ° to 2' 0 C.), ‘ hc 
minutes a cert a' A ^ * 1?St ^ ° C Evcn at the end of five 
noticeable in n C ^ ree cd agglutination of thc red cells was 
° me ° f thc tubes w hich had been placed in the ice 
Tm. dt, -—-—- --. 
