534 
Table I.—Examination of Sleeping Sickness Serum for Auto- and Iso-agglutinin 
Equal volumes of serum and red blood cell 
suspension used. 
5 per cent, suspension 
of washed erythrocytes 
in normal saline 
solution 
Source from which 
serum was 
obtained 
Temp. 
Result 
°C. 
Sleeping Sickness . 
Sleeping Sickness . 
37 
No agglutination 
in 30 mins- 
r8 
Marked 
30 ,. 
o 
Complete 
10 ,. 
Normal individual A ... 
Sleeping Sicknes- . 
37 
No agglutination 
in 3c ,. 
18 
Complete „ 
3 ° 
o 
*1 11 
JO „ 
Normal individual 11 ... 
Sleeping Sickness . 
37 
No agglutination 
in 30 „ 
j8 
Marked 
30 n 
o 
Complete 
10 ,. 
Normal individual C ... 
Sleeping Sicknes* . 
37 
No agglutination 
in ;o „ 
iS 
Marked 
30 
o 
Complete 
10 „ 
Normal individual C ... 
Normal individual C . 
37 
No agglutination in 30 „ 
1 ,s 
1? 1? 
3 ° .. 
o 
11 11 
30 ,, 
Normal individual C ... 
Normal individual 11 . 
37 
No agglutination in 30 ,. 
iS 
11 1? 
30 „ 
1 
o 
Marked 
3 ° n 
SPONTANEOUS AGGLUTINATION OF THE RED CELL 
SUSPENSIONS 
On laic occasions it was found that the 5 per cent, suspension 
. UCeds w ki c h had been washed three times in large volumes 
9 pci cent, sodium chloride solution underwent a spontaneous 
Ul,natlon 111 the entire absence of serum. Indeed, in one or two 
where the animals exhibited an extreme degree of 
agglutination, some difficulty was experienced in obtaining an 
uspension of the erythrocytes. ] he probable explanation of 
'1 ontaneous agglutination is that it was due to the absorption 
" agg utinm from the plasma by the red cells immediately aftei 
16 bl ° 0d Was shed the cold citrated saline solution. 
