535 
This difficulty was obviated by collecting the blood in warm 
citrate solution, and then rapidly centrifugalising and decanting 
off the citrated plasma. The red cells were then washed thrice in 
warm normal saline solution. Suspensions prepared in this way 
exhibited no tendency to spontaneous clumping. 
It might be mentioned in this connection that Klein* has 
succeeded in obtaining agglutinating solutions by grinding up with 
quartz sand the well-washed erythrocytes of certain animals (rabbit, 
dog, hen, and guinea-pig,. These extracts sometimes agglutinated 
the red cells of other animals, and frequently also the erythrocytes 
of the same kind of animal, and even those of the same animal. 
ABSORPTION OF AGGLUTININ BY RED CELLS 
Experiment .— To one volume of the citrated plasma of Rabbit 
896 (infected with T. dimorphon , which caused great agglutination 
when added to its own red cells and to those of normal rabbits, 
were added five volumes of the undiluted well-washed red cells of 
the same animal. The mixture was then divided into two equal 
portions, A and B. A was placed in the incubator at 37 0 C. and B 
in the ice chest at o° C. At the end of three hours the extracted 
plasmas were separated from the red cells by centrifugalisation, 
and were examined for auto- and iso-agglutinins. 
Table II. 
Equal volumes of extracted plasma and erythrocyte 
suspension used. Temp, of experiment o° C. 
Result 
5 per cent, suspension of 
washed erythrocytes in 
normal saline solution. 
Extracted plasma 
Rabbit 8q6 . 
A 
» 
A 
B 
Complete agglutination in 10 mins. 
No agglutination in 60 mins. 
Complete agglutination in 10 mins. 
Slight agglutination in 60 mins. 
1 
Normal rabbit . 
The plasma which had been in contact with the redl cells at o° C. 
had almost completely lost its agglutinating action, whilst the other 
. ''Btimsa zur Keuntni.s dor Agglutination rothK Blutterpercheo,’ Wien. Klin, ttodi 
1902, No. 16, p. 41 3. 
