54 § 
Table VI.—Agglutinating Action of a Solution obtained by Digesting Auto-agglutinated Red 
Blood Cells with Normal Saline Solution at 40° C. 
Equal volumes of red cell suspension and defibrinated 
plasma or digest solution used. Temperature of 
experiment o° C. 
5 per cent, suspension 
of washed erythrocytes 
in normal saline 
solution 
Untreated defibrinated plasma 
of Rabbit 1035 (infected with 
nagana), and solution 
obtained by digesting the 
auto-agglutinated red cells of 
the same animal with normal 
saline solution at 37 0 C. 
Result 
Rabbit 1035 (infected with 
T. brucei) 
Normal Rabbit A ... 
Donkey 2 (infected with 
T. rbodesiense) 
Normal Donkey A 
Goat 1041 (infected with 
T. rbodesiense ) 
Normal Goat A 
Rat 
Guinea-pig 
°og 
Horse 
Macacus rhesus 
Cercopilbecus callitrichus .. 
Human being 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution 
Defibrinated plasma 
Digest solution 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Defibrinated plasma 
Digest solution ... 
Complete agglutination in 10mini. 
Slight 
Complete 
No 
Complete 
No 
Complete 
Partial 
Complete 
10 „ 
10 „ 
to „ 
10 „ 
3 ° .. 
»5 n 
45 « 
10 „ 
45 .. 
10 „ 
45 .. 
»5 » 
20 „ 
'5 
20 ,, 
5 » 
10 „ 
10 „ 
45 is 
*5 '■ 
30 „ 
>5 « 
15 .. 
to ,, 
15 .. 
dumped'red 1, rtiu° ur s P ec .“ nen * of . normal saline solution which had been used for washing the 
■ssk't.'ta s.’ssi wi,h ,ht «—»*■ **■ » 
1 lie information obtained from observations of this kind is 
tremeh interesting. In the experiment recorded a substance was 
extracted from the auto-agglutinated erythrocytes of a rabbit 
m ^ brucei which clumped not only its own erythrocytes 
ose of other rabbits, but also the red cells of many other 
.. different species. In other words it would appear that 
agglutinin is not a body equipped with a high degree of 
