1210 Wisconsin Academy of Sciences, Arts, and Letters. 
tests could be employed. The zygospores were dissected from 
their black coats, mounted in water and crushed under a cover- 
glass. The oil exuded in the form of yellow globules, while 
the amorphous substance was forced out more slowly and in a 
more or less irregular mass. Sudan III was then added; some¬ 
times the zygospores were mounted directly an this reagent and 
then crushed. The oil became a brilliant red while the other 
substance remained clear. If the Sudan III were followed by 
chloroform, the oil globules immediately disappeared leaving 
the amorphous mass behind. The addition of ammonia water 
caused the latter to disappear at once. Sodium hydrate had 
much the same effect but acted more slowly. The xantho-proteic 
test was applied to other crushed zygospores and this mass took 
on a decided orange color. Millon’s reagent failed to give the 
color changes. Zygospores were crushed in alkaline phenol- 
phthalein and apparently gave an acid reaction, since the por¬ 
tion of the reagent through which the zygospore content was 
forced lost its color. The significance of this, however, is not 
great, because the reaction of the rest of the cytoplasm may 
have been acid. On the other hand, the cytoplasm and nuclei 
at this time are reduced to a thin parietal layer, as shown in 
sections (PL III, fig. 17), so the acid reaction was probably 
caused by these amorphous inclusions. Since it was impracti¬ 
cable to attempt to isolate this substance in question, the con¬ 
clusions based upon these tests can only be of the most tenta¬ 
tive nature but the reactions appear to be those of the nucleo- 
proteins. These tests have served merely to separate this type 
of reserve material from the oil which is present and to aid in 
the interpretation of a rather perplexing condition existing in the 
mature zygospore. 
At the time that the nuclear disorganizations begin, there 
appears a marked zonation in the zygospore (PI. II, fig. 12). 
The inner coat of the zygospore, which is semi-transparent, is 
produced at this time. It is very thick and resistant. It is 
laid down within the brown, roughened wall previously formed. 
Thus the mature zygospore is invested with two thick coats 
(PI. Ill, fig. 14) and parts of the original gametophore walls 
may still be found on the outside in many places. Toward the 
margin of the zygospore there appear bluish-staining areas. In 
the earlier conditions they appear merely as vacuoles in which 
