1216 Wisconsin Academy of Sciences, Arts, and Letters. 
10. Grouping of the nuclei follows. There occur from 12 to 
16 nuclei in each group. 
11. Nuclear fusions in pairs follow, resulting in the reduction 
in the number of nuclei and in an increase in the size of the 
nuclei. 
12. Deep-staining, crystal-like bodies are to be found at all 
stages. They resemble the crystalloids and globoids of the 
higher plants and undoubtedly constitute a form of reserve sub¬ 
stance. 
13. Zonations within the zygospore take place at the time 
of the formation of the second wall which invests the zygospore, 
and at the time of the appearance of the oil masses. 
14. Disorganization of some of the nuclei follows and re¬ 
sults in the formation of a large amount of irregular to globular 
masses of a deep-staining substance. 
15. The oil plastids enlarge, show reduction in number and 
appear as definite reticulate bodies. 
16. In the mature zygospore there are found two different 
types of reserve material. 
a. A large amorphous mass of what is probably nucleo- 
protein substance. 
b. A considerable amount of oil. 
17. Nuclei of the typical form are found in much reduced 
numbers in zygospores six months old. The protoplasm is re¬ 
duced to a thin parietal layer. 
This work has been done under the direction of Professor G. 
M. Reed of the University of Missouri and I am greatly in¬ 
debted to him for his unfailing interest and valuable sugges¬ 
tions. 
Madison, Wisconsin. 
