112 
EIGHTH REPORT. 
plates made from the heart’s blood. Within another twelve hours these 
plates have developed and cultures are made from the colonies. For these 
plates litmus agar is used for the purpose of distinguishing between 
bacteria of the colon group and those of the typhoid group. Moreover 
the colonies on the plates made from the heart’s blood are grown in 
special media for the purpose of more exact differentiation. However, 
I will not go further into this point in this paper. 
While this bacteriological investigation is being carried on, the ordi¬ 
nary chemical analysis is made. 
The cities and villages of Michigan are supplied with (1) well water, 
(2) waters from small lakes, (3) waters from the great lakes, (4) waters 
from streams, and (5) waters from springs. 
WELL WATERS. 
The following table gives the results of the examinations of well 
waters: 
TABLE I. 
Well Water and City Waters from Wells. 
Place. 
No. 
sample. 
Ammonia. 
Cl. 
No. of 
Germs. 
Effect on 
animals. 
Free. 
Alb. 
3 
.282 
.256 
32. 
121 
6 
.123 
.096 
16.7 
2,857 
4+ 
Ann Arbor. 
15 
.420 
.065 
14.8 
502 
14 4- of 36 
1 
.100 
.170 
9.2 
1,560 
1+ 
Amasa. 
5 
.126 
.238 
2.6 
702 
1+ 
5 
.226 
.122 
14.58 
1,960 
3+ 
1 
.220 
.096 
8. 
400 
5 
.130 
.101 
6.65 
3,985 
1 + 
13 
.180 
.084 
8.7 
1,894 
6 + oflO 
1 
.080 
14.8 
300 
1 
.030 
.050 
9.9 
100 
7 
.111 
.078 
10.1 
540 
1 + 
1 
.206 
.084 
4.5 
105 
1 
.19 
.12 
6. 
10 
1 
0.9 
.22 
16.5 
1 
.01 
.01 
33. 
5 
4 
.007 
.035 
17.5 
2,225 
1 
.008 
.004 
4. 
1,500 
Deerfield. 
1 
.508 
.158 
695 
898 
1 + 
Dowagiac. 
2 
.018 
.115 
1.8 
47 
14- 
2 
0.68 
.089 
23.37 
2,920 
3 
.045 
.122 
14.8 
130,025 
i+ 
1 
.005 
.062 
• 9.9 
22 
3 
.016 
043 
30. 
1,560 
Eloise. 
1 
.130 
.120 
37. 
16,680 
14- 
Flint. 
1 
.011 
040 
5. 
100 
14- 
2 
568 
Flushing. 
6 
.012 
.080 
35.9 
870 
34- 
Grand Rapids. 
11 
007. 
.115 
12.2 
597 
1+ 
1 
.374 
.044 
11.8 
63 
5 
.030 
.410 
7.5 
17,770 
24- 
Greenville. 
1 
1.46 
.40 
38 
550 
14- 
1 
.001 
.001 
9. 
300 
Gregory.;. 
1 
.050 
.060 
11 
5.740 
14- 
2 
6,150 
24- 
1 
.20 
.11 
18. 
850 
1 
.01 
.01 
53 
2,500 
7 
.217 
.111 
21.1 
182 
14- 
6 
.083 
.260 
19.6 
1,707 
Holt..... 
1 
.030 
.050 
24.7 
2,020 
