12G 
edge of a wood at the top of the banks ol a small stream. W. T. Swin¬ 
gle, No. 4027, October 10, 1891, Still Pond, Kent County, Md. With 
mature oospores in leaves. This number includes leaves from two in¬ 
dividuals of a rough-leaved, coarse-twigged form of the host, on which 
the fungus was rare. One individual was a good-sized tree, growing 
with those from which No. 4026 was collected; the other (No. 4027a) was 
a small sapling growingby the wayside. 
42. Peronospora echinospermi Swingle. 
1889. Peronospora Cynoglossi Burrill, var. (?) Echinospermi Swingle. A list of Kan¬ 
sas species of Peronosporacecse No. 21. <Trans. of 20 and 21st meetings Kaus. 
Acad. Sci. (1887-1888), Yol. xi, pp. 77-78. 
Conidia and oospores on leaves, stems, and fruits of Borraginacese. 
On Echinospermum EedowsUi Lehm., var. cupulatum Gr. E. Bar¬ 
tholomew, No. 469, June 10, 1891. Rockport, Rooks County, Kans. 
Conidiophores on leaves, stems, and calyces; oospores in inesophyll of 
leaves, bark, parenchyma, and pith of stems and in walls of nutlets. 
On Echinospermum Redowsldi Lelim., var. occidental Wats. F. W. 
Anderson, No. 10, May 27, 1887, Helena, Lewis and Clarke County, 
Mont. Conidia only on leaves. E. Bartholomew, No. 470, June 15,1891, 
Rockport, Rooks County, Kans. Conidia on leaves, calyces, and stems; 
oospores, bark, parenchyma of stems, and walls of nutlets. 
Through the kindness of Mr. Bartholomew I have been able to ob¬ 
tain an abundance of mature oospores of this form, and after a careful 
study I feel reasonably certain that it is specifically distinct from P. 
cynoglossi Burrill, to which I referred it as a doubtful variety in 1889.* 
The oospores occur in great profusion in the cortical parenchyma of the 
stems, and occasionally beneath the thickened outer layer in the wall 
ot the nutlets, in mesopliyll of leaves and calyces and in pith of stems. 
It is noticeable that they occur only in thin-walled tissue, and of such 
tissue they seem to prefer the best nourished. The bark and the seeds, 
which contain nearly all the oospores, resist decomposition longest after 
the death of the plant. 
The oospores are unusually large, in fact the largest I have studied, 
being 35 to 50 by 33 to 47 //, mostly 38 to 45 by 36 to 42 /<, in diameter. 
They are rather dark-brown, nearly globose, have a thick (mostly 2 to 
7 /*), brown epispore, whose surface is slightly undulating and furnished 
with curious markings. 
These epispore markings consist of very narrow (one-eiglith to one-lialf 
iiregular, interrupted zigzag lines, which occasionally anastomose.. 
The lines do not appear to be raised markings, such as are common on 
oospores of Peronosporacece , but rather lighter colored portions of the 
epispore Avail. 
In sections the epispore has a distinctly radiate structure, seeming to 
be composed of brown, radiating prisms, between which are narrow 
stripes of lighter-colored substance. 
A list of the Kansas species of Peronosporaceae loc. cit. I wish to correct an error 
onp. 77 of this list. The host plant of P. cynoglossi should he Cynoglossum Virgin- 
icum L.j not C. officinalis L. ; as given. 
