230 
or May. All of them showed symptoms at once in all parts, and all 
died in August. There were and have been no cases of rosette in the 
rest .of the young Elbertas (about 4,000); there was only one case in 
that part of the orchard joining this nursery, and there were less than 
last year in the other orchards on this farm, i. «., about 27 in a total of 
10,000 trees. This makes it overwhelmingly probable that the results 
here detailed are to be ascribed to the inoculations and not to any out¬ 
side influence. 
This experiment is especially interesting for a number of reasons: 
(1) The disease has now been communicated from artificially in¬ 
fected trees to healthy ones, i. e., the infection has been carried a sec¬ 
ond remove from the orchard trees which were its original source. 
(Bull. No. 1.) 
(2) The rosette can be communicated from root to root as well as 
from stem to stem. 
(3) The root inoculated trees did not develop symptoms as soon as 
those which were inoculated above ground the preceding year, prob¬ 
ably because the contagion had a longer distance to travel through the 
tissues. 
(4) The small per cent of infections in comparison with the results 
ot .1891 (Bull. No. 1) is attributable to the smaller number of unions. 
There were unions on only 12 trees at most and the disease followed 
in every case where from one-half to the whole of the graft became 
firmly united to the root. 
(5) In case of the five doubtful unions the grafts came from as many 
different trees, and it is possible that these fragments may not have 
contained the infectious material even if any part really united with 
the roots, which is also a matter of doubt. 
(G) In the other 52 trees, as in 4 trees of Experiment 1, (Bull. No. 1), 
simple contact failed to induce the rosette, although in all cases the 
diseased tissue (young prosenchyma and pericambium) was bound 
down tightly on to the meristem of the root, and in several instances 
was found to have been inclosed and tightly squeezed, and even deeply 
buried between the growing tissues of the root. 
YI. Inoculations of young peach trees with micro-organisms derived 
from cultures .—These experiments were made to determine whether 
micro-organisms were constantly or commonly present in diseased tis¬ 
sues, and whether pure cultures of any of them would induce the 
rosette. Numerous tube and plate cultures from rosetted trees were 
made at Griffin, Ga., by W. T. Swingle and myself, in the summer of 
1891, with as great care as our limited facilities would permit. A 
number of interesting yeasts and bacteria were isolated from the 
tissues or appeared in the cultures as contaminations. Notes were 
made on the manner of growth and microscopic appearance, stained 
and unstained, of all these forms—about twenty—and pure cultures 
from the original colonies were used for purposes of inoculation. 
