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A series of 20 young trees was inoculated on the farm of H. W. 
Hasselkus, east of Griffin, and a duplicate series was inoculated on the 
farm of J. I). Husted, at Vineyard. Some of the more promising organ¬ 
isms, e. g ., those which grew but feebly on the agar or gelatine, and 
those which occurred in the cultures most frequently, were also inserted 
into a row of young trees in the garden of Mr. Hasselkus in Griffin. 
Each tree was inoculated on the main axis above ground in three places 
and in three slightly different ways, as follows : 
(1) A T-shaped slit was made through the bark, and one fUvp was 
separated from the cambium and slightly lifted. A mass of the organ¬ 
isms was then removed from the culture on the loop of a platinum wire 
and inserted into the wedge-shaped cavity between the wood and bark. 
The latter was then bound securely in place. 
(2) Into a similar slit a bud cut from the same tree was inserted and 
bound in place as in ordinary budding, the inner bark of the bud hav¬ 
ing first been carefully smeared with the micro-organisms, so that bark 
of insert and wood of stock were brought into close contact with a thin 
layer of germs between. 
(3) The third inoculation was made in the same Avay, i. c., the inner 
surface of the insert was smeared with the germs, but the wood of the 
bud was not removed. 
The platinum wire was flamed before each inoculation, and the work 
was carried on as rapidly and deftly as possible to avoid contamina¬ 
tions. The inoculations were made in Mr. Hasselkus’s yard June 15 
and 16, on his farm June 19, and at Mr. Husted’s place June 22, 1891. 
These trees were examined in the fall of 1891, and again in the fall of 
1892. None of them developed rosette or any symptoms suggestive of 
it. In some cases there was considerable swelling and flow of gum at 
the points of inoculation, but none of the trees died or became sickly. 
All of the trees made a good growth, and those belonging to Mr. Has¬ 
selkus grew enormously. Of course many of the buds were thrown out, 
but others healed on in spite of the coating of micro-organisms. 
An experiment was also tried using scrapings and bruised fragments 
of diseased tissues as infective material, but it was in old trees on a 
small scale, and the results are not conclusive enough to make it worth 
reporting. 
III.— CONCLUSIONS RELATIVE TO THE NATURE OF THIS DISEASE. 
(1) Excluding a few doubtful cases, the disease was conveyed from 
peach to peach whenever an organic union took place between the dis¬ 
eased buds and the healthy stocks (two experiments—128 trees). 
(2) In no case was the disease transmitted artificially by mere con¬ 
tact even when meristem was bound very closely to meristem (two ex- 
periments—56 trees). 
(3) From the failure to induce rosette by simple contact it is prob- 
