353 
sections was placed in one of four grades as regards injury to the foliage 
the record stood as follows: 
Treatment. 
Number 
of seed¬ 
lings. 
Number 
in grade 
1. 
Number 
in grade 
2. 
Number 
in grade 
3. 
Number 
in grade 
4. 
Average 
grade. 
Mixture No. 13. 
31 
9 
17 
4 
1 
1.9 
Control on No. 13. 
25 
2 
12 
3 
8 
2.6 
Mixture No. 25. 
26 
0 
20 
4 
2 
2.3 
Control on No. 25. 
31 
0 
11 
9 
11 
3.0 
The beneficial effects of the treatments with mixture No. 13 were 
plainly evident, and of the twelve mixtures employed this is the only 
one promising good results. The second row was divided into six sec¬ 
tions, sections 1 and 2 being treated five and six times, respectively, 
with ammoniacal solution, and sections 4 and G on the same dates with 
Bordeaux mixture. The dates of treatment and the formulae for the 
mixtures are given on a previous page, and were the same as those for 
the budded stocks treated in 1892. Sections 3 and G were intended to 
serve as controls, but unfortunately, by mistake, section G received one 
treatment on June 1G with Bordeaux mixture. The beneficial effect of 
the Bordeaux mixture was evident, as the disease spread upon the 
untreated sections, but the ammoniacal solution was plainly injurious to 
the foliage, the leaves which were treated with this assuming a faded 
brownish hue. In October the difference between the untreated stocks 
and those sprayed with Bordeaux mixture was marked. The former had 
lost many of their leaves and had few perfect ones, while the latter were 
provided with almost perfect foliage. No marked difference between 
five and six treatments was observable. The preventive effect of 
ammoniacal solution, while apparent, was, in the sections treated five 
and six times, inferior to that of Bordeaux mixture. 
On October 15 the earth was removed from the base, and the seed¬ 
lings of sections 3, 4, and 5 were calipered at the collar. The following 
data were thus obtained: 
74 seedlings untreated.-. 
57 seedlings treated 5 times with Bordeaux mixture 
75 seedlings treated 6 times with Bordeaux mixture 
As is shown, no difference in diameter of treated and untreated seed¬ 
lings was observable. It is hoped that another year’s observations upon 
the stocks may be made to ascertain the effect of maintaining the foliage 
upon plants like the horse chestnut, Avhich make their principal growth 
before the disease defoliates them. This preliminary experiment, how¬ 
ever, certainly warrants the recommendation of Bordeaux mixture as a 
preventive of horse-chestnut leaf-blight. 
Average 
caliper in 
of an inch. 
12.8 
12. G 
12.6 
