Colorado Hays and Fodders. 
17 
The hay was passed through a cutter and the sample taken 
by Professor W. W. Cooke, who was at the time Professor of Agri¬ 
culture at this institution, and by him delivered to me. The hay 
was discolored to a degree which might be produced by its being 
exposed to a heavy dew or a light rain. An analysis of it indi¬ 
cated it to be at least a fair quality of hay, the only thing attract¬ 
ing attention being the very unusually low percentage of ether 
extract or fat. 
Two things were possible in our results: We might have ob 
tained too low results in our analysis of the hay, or those obtained 
for the fat in the feces might have been too high, and it is con¬ 
ceivable that both determinations might have been wrong, even 
though the former was made in duplicate and the latter was the 
average of five closely agreeing determinations. The analysis of 
both the hay and the feces were repeated without changing the 
results. It was then thought the alfalfa being very rich in chlo¬ 
rophyll, the coloring matters might have accumulated in the feces 
and possibly, having been rendered more readily soluble in ether, 
might account for a part of the discrepency between our results and 
thoseof others. The hay and feces of sheep Nos. 3, 4and 8 were re¬ 
sampled, the samples carefully dried in hydrogen and extracted 
with petroleum ether, boiling from 35 deg. to 50 deg. Pe¬ 
troleum ether of this boiling point dissolved about 50 per cent as 
much out of both hay and feces as the anhydrous ether. The pe¬ 
troleum ether Bp. 35 deg.-50 deg., dissolved 1.78, 1.97 and 1.78 
per cent from the feces, whereas the anhydrous ether dissolved 
3.64, 3.62 and 3.62 per cent. The petroleum extract had a yel¬ 
lowish green color and it was evident that there was some color¬ 
ing matter present which was freely soluble in this menstruum. 
An attempt to separate the fatty acids and in this manner to 
eliminate the question of coloring matters and bile products, gave 
unsatisfactory results. 
We next tried a higher boiling petroleum, 50 deg.-60 deg. 
We found this much more difficult to work with than the lower 
boiling petroleum, and further, that it yielded a much higher per¬ 
centage of extract, in one instance falling only 0.10 percent below 
the ether and in no instance more than 1 per cent^ less 
than the ether. After extracting five samples with petroleum Bp. 
50 deg.-60 deg., we abandoned it and had recourse to alternate 
extraction with petroleum Bp. 35 deg.-50 deg. and anhydrous 
ether, also treating same samples in reverse order. As a result of 
this treatment we found that samples treated with ether yield but 
little, 0.07 per cent average of three trials, to petroleum Bp. 35 
deg.-50 deg., while those treated with petroleum Bp. 35 deg.- 
50 deg. yield 0.90 per cent to the ether. At first I supposed that 
this difference was due to chlorophyll soluble in ether, but insol- 
