Actinomyces-like Endotrophic Mycorhiza . 41 
Hyphas growing into some of the parenchymatous cells form a 
network round nucleus; locally they swell into little club-shaped 
masses (“ massues ”). These are either directly inserted on the 
hyphae, or (as may occur in the rhizomes), terminate short lateral 
branches (Fig. 5, ct). 
The hyphae are acid fast and retain a red colour after 
coloration by carbol fuchsin and the Ziehl-Niellsen method. The 
“massues’’are basophilous—and more so as they age; they are 
“gram positive,” and stain a deep blue with cotton blue. 
Fig. 2. From same section as Fig. 1. N. Hypertrophied nucleus of 
parenchymatous cell. c. club shaped “massues.” h. hyphae (uncoloured 
by alcohol). 
Fig. 3. Nucleus of infected cell, showing detail of infection and 
relative dimensions of endotroph. (Oil imm. obj, x l 5 , oc. 9). 
N. Nucleus, c, full grown “massues,” (gram-positive) d , young 
“ massue,” h, hyphae. 
The nucleus in infected cells enlarges or may divide; the 
nucleolus becomes larger and basophilous. The whole of the 
nuclear material becomes granular and retains basic dyes, whereas 
the nuclei of the epidermal cells are acidophilous and stain deeply 
with eosine. 
No hypertrophy of infected cells was observed, but hyperplasia 
may result from division by irregularly placed walls. Cytoplasm 
may shrink in neighbouring non*infected cells, which brings the 
nucleus into contact with the cell wall (Fig. 1). 
