. 106 
W. ZUILL. 
PATHOLOGICAL ANATOMY AND RESUME OF MACROSCOPY AND MICRO¬ 
SCOPY OF THE LESIONS. 
From the foregoing record, and from the above observations, 
(as illustrated by my own microscopic examination) the following 
may be said in relation to the pathological anatomy of this dis¬ 
ease. There are no distinctive or characteristic lesions found on 
external examination of the dead animal, such as would give any 
definite idea of the cause of death, or of the nature of the disease. 
The only prominent symptoms however, are great distention of 
the belly, frothing at the mouth, and a purulent discharge from 
the nostrils. The blood is found to be dark or black in color, in 
a fluid or semi-fluid condition, or in the larger blood vessels, may 
form soft friable clots, and is loaded with carbonic acid gas. The 
microscopical examinations, which I made of the blood of animals 
suffering from this disease, were conducted in the laboratory of 
the University of Pennsylvania. Some of the examinations 
were made from animals that had perished of the disease ; others 
were made from the blood taken during life by bleeding at the 
ear; in other instances the blood was taken while the animal 
was being slaughtered. 
Extreme care was taken to prevent contamination from the 
air or surroundings. The method of collection and examination 
were as follows: Sometimes I collected the blood into sealed 
tubes, by introducing the sealed extremity of the tube into a 
vein, and breaking off the end of the same within the vessel, in 
this way the blood was drawn into the tube without the least 
possibility of contamination ; it w T as then at once sealed. At other 
times the fresh blood was smeared upon glass covers and rapidly 
dried. In this dry condition it can be preserved for an indefinite; 
time in clean boxes. If any extraneous matter should fall upon 
covers prepared in this way, it would at once be recognized as for¬ 
eign to the dried mass upon the cover, as it would be seen in a differ¬ 
ent focus, under the microscope, being located superficially. Be¬ 
sides all extraneous germs, or foreign matter, can be easily washed 
off from the prepared glass covers. The blood thus dried upon 
these covers was then treated with analine dyes, precisely after 
the method for staining sputum. For the first dye, analine blue 
