218 
W. ZUILL. 
CONTAGIOUS PLEURO-PNEUMONIA. 
Thesis presented by W. Zuili., D.V.S., before the University of Pennsylvania, 
Medical Department. 
INQUIRY INTO THE MORPHOLOGY AND CHARACTER OF THE MICROBE INFEST¬ 
ING THE LYMPn SPACES tn PLEURO PNEUMONIA. 
{Continued from page 165.) 
I have already referred to the microbe which I detected in the 
lesions of pleuro-pneumonia, and in the following pages I shall 
give some culture experiments which I made with this microbe. 
In reference to this part of my investigations, the following notes 
were taken: 
Saturday, October 6, 1883.—To-day a piece of lung and some 
serum were taken to the University of Pennsylvania for micro¬ 
scopical examination, and to make culture experiments, with the 
view of determining whether or not any organisms were present, 
and if so, to study the nature and character of the microbe. 
The microscopical examinations of the serum showed numerous 
lymph corpuscles, a great deal of fibrine, and admixture of red 
blood corpuscles ; there were also present large clouds of spherical 
bacteria microbes. The absence of bacteria thermo indicated 
that no putrifactive changes had taken place. A piece of the 
lung was also examined in glycerine, and it showed the same 
microbes as were found in the serum, besides the elements of the 
lung tissue. In preparing for the culture experiments carefully 
sterilized tubes were taken, which were partly filled with pure 
sterilized gelatine. All instruments used in connection with the 
operation were also subjected to high degrees of heat, for the pur¬ 
pose of destroying all germs. After all these necessary precautions 
had been taken, a very small piece of lung tissue, cut from the 
centre of the piece of fresh lung which was at hand, and with 
carefully steralized instruments, was placed in a number of the 
tubes. Great care had been taken to preserve the lung tissue, from 
which the experiments were made, from infection from air. Imme¬ 
diately on the removal of the section from the lung, it was placed 
on a fold of cotton cloth, soaked with a solution of the bi-cliloride 
of mercury, 1^ grains to the ounce; this was folded around the 
piece of lung five or six times, and then carefully wrapped in 
