contagious pleuro-pneumonia 
219 
waxed paper, and again wrapped in cotton cloth treated as before, 
followed by waxed paper folded four or five times, and in this way 
taken to the laboratory. Contamination from air was absolutely im¬ 
possible. Other tubes were inoculated with the pure serum from 
the lung, which was collected into vacuum tubes, by first inserting 
the point of the tube beneath the pleura of the organ, deep into 
the distended lymph spaces, and there breaking off the point, when 
the tube will immediately fill with serum, thus avoiding the 
slightest possibility of the entrance of air. As soon as it was 
full it was immediately sealed by the blow-pipe and alcohol flame, 
and in this way the serum was obtained absolutely pure. This 
serum was also used for culture purposes. Monday, October 8, 
1883—It was seen that germs had begun to develop in the culture. 
October 9.—Germs continue to grow; development to a much 
greater extent than yesterday. October 10.—Culture continues to 
develop; liquefection is taking place. October 11.—The culture 
medium is nearly full of new growth, liquefaction has gone on, 
and a peculiar greenish-blue color invades the upper part of the 
culture medium. October 12.—The cultures were found to be 
very impure, as far as external appearances were concerned; that 
is to say, to contain more than one form of fungi, except two of 
the cultures in salt dishes, which showed development of the 
microbes only as a precipitate. This precipitate was marked in all 
the cultures. With the exception of the two salt dishes, all con¬ 
tained a .micoderma, that is, a development of fungi at the 
periphery. The development of the precipitate fungi had a marked 
effect in liquifying the jelly. This was the more apparent, as two 
of the cultures, which showed little or no liquifaction, showed 
also less development of the microbes. There was no distinct 
difference in the cultures made from the serum and from the lung 
tissue, except that those from the serum appeared to give a purer 
culture than those from the lung tissue. October 13.—Micro¬ 
scopic examination of the culture showed that oideiun existed in 
spores, and also in mycelium; large quantities of bacterium 
thermo could be seen. It was now found that our jelly was im¬ 
pure, which will make it necessary to go over the same ground 
again. 
CD 
