220 
W. ZUILL. 
January 5, 1884.—After a number of attempts to obtain pure 
chicken soup for a culture medium, I at last succeeded. Having 
obtained a chicken of medium size, removed the flesh free from fat, 
bones and skin, it is then placed in a vessel of suitable size with 
sufficient water to make about sixteen ounces of soup, which is to 
be strained and filtered through a wet filter, which removes all the 
fat. This makes a beautiful, clear, amber-colored liquid, which 
must be placed into a Florence flask, and sterilized over a water 
bath at the boiling point. It is necessary to repeat this four or 
five times, in order to be sure of its purity. Having satisfied 
myself that the • soup was pure, I proceeded to fill the glass bulbs 
which had been previously made. The serum used in this experi¬ 
ment was drawn into a vacuum tube, from beneath the pleura of 
the diseased lung, immediately on its removal from the body of 
the animal. This serum was mixed with a few drachms of the soup. 
The bulbs previously filled and sealed, were now opened, slightly 
warmed and a few drops of the contents forced out. The open end 
being now placed in the inoculating fluid, it replaces that which 
was forced out, and in this way as many bulbs as are desired can 
be inoculated. Here again great care was taken to prevent con¬ 
tamination from the air. The bulbs are now placed to one side or 
in the incubator, that the germs may grow. January 9.—In¬ 
spected cultures. They did not look very encouraging and were 
left for future examinations. Not having the success with these 
cultures that I expected, I find that I can go no further with my 
research at present, and I am very sorry that my time does not 
permit me to go into the investigation more exhaustively. I am 
not satisfied with my cultures, as I could not carry them on for 
a sufficient length of time, or a sufficient number of generations. 
My original intention was to inoculate animals with the isolated 
microbes; I have, however, given this up for the present, although 
this would have been a very important matter, to test the patho¬ 
genetic properties of the cultivated microbe. I therefore do not 
pretend more than to have demonstrated the constant presence of 
a microbe in the lesions, the blood, and serum, and which can be 
isolated and cultivated. The cultures of pleuro-pneumonia I still 
continue and I hope to be able to make some inoculation experi¬ 
ments on calves during the coming spring. 
