Notes. 
1 55 
The general relations between the sexual organs is similar to that described by these 
authors. The antheridium, which is usually terminal, but may be intercalary, is 
penetrated by the oogonial branch, which then enlarges above to form the oogonium. 
It is of interest to record that in ripe oogonia, obtained from old desiccated pods, 
the antheridia become readily detached, and then show that their walls are intact, and 
that a definite antheridial membrane surrounds that of the stalk of the oogonium. 
This might be expected from a study of the early stages of penetration, but it is 
impossible to distinguish the double membrane in sections of the mature oogonium. 
The material so far available has not been suitable for cytological work, but 
A B C D 
Fig. 2. a, B, c, oogonia from cells of mesocarp of cocoa pod, showing the numerous sacculae 
and amphigynous antheridia ; D, section of oogonium with ripe oospore; e, oogonia with detached, 
antheridia found in desiccated material three and a half months old. an ., antheridium ; og., oogo¬ 
nium ; sp., oospore, x 6oo. 
preliminary observations show that the young oogonium is multinucleate, that little 
or no periplasm remains after the delimitation of the oosphere, and that the oosphere 
and ripe oospore are uninucleate. The oospore has a comparatively thin wall with 
little or no epispore. All attempts to germinate the oospores have so far failed of results. 
That a definite relation existed between these sexual organs and the conidial- 
bearing mycelium, was evident from their constant association and from the fact that 
healthy pods, infected with the conidia, showed subsequently the presence of oogonia. 
Any doubts existing have, however, been set at rest by tracing the continuity of hyphae 
bearing conidia with those bearing oogonia, in mycelia from artificial cultures. 
These were tube cultures in which both sterilized cocoa pod and a cocoa pod agar 
were employed. 
Up to the present no sexual organs have been found either in the tissues of the 
coffee pericarp or in artificial media prepared from coffee fruits. 
