Physiology of the Genus Eidamia . 407 
described in detail elsewhere it is unnecessary to make more than brief 
reference to this subject here. The growth experiments were carried out in 
50 c.c. flasks containing 25 c.c. of a 1 per cent, neutral solution of practically 
pure pectin. The amount of pectin actually present in each flask was 
estimated after autoclaving. The flasks were inoculated in duplicate series 
and kept at 20° C. for a given period. At the end of this period final 
pectin estimations were made, the solutions were titrated against standard 
alkali and tested with Fehling’s solution for the presence of reducing sugar. 
The following general results were obtained: 
E. acremonioides. No growth. 
E. catenidata. Moderate growth with utilization of pectin. Solution 
slightly acid. Liquid does not become green. Fehling’s solution not 
reduced. 
E . viridescens. Moderate growth. Pectin completely used up. Solu¬ 
tion slightly acid. Liquid becomes greenish in colour. Fehling’s solution 
reduced. 
4. Peptone. 
Flasks, containing a 2 per cent, solution of peptone with an initial 
pH 6-8 and total acidity equivalent to 10 c.c. N/i NaOH per litre, were 
inoculated with E. catenidata , E. viridescens , and E. acremonioides and 
kept at a temperature of 20° C. After fourteen days the growth character¬ 
istics were recorded, the final pH was obtained by the indicator method, the 
total alkalinity estimated in terms of c.c. N/i HC 1 usingneutral red asindicator, 
and the atmosphere of the flask tested for the presence of ammonia by 
means of suspended litmus paper. 
Re stilt : 
E. catenidata. Moderate floating and submerged growth: superficial 
growth with a fine, white, mealy appearance: both conidia and macro¬ 
spores present. Solution alkaline to litmus and suspended red litmus paper 
blues slowly. pH 7*8. Alkalinity equivalent to 5-8 c.c. N/i HC 1 per litre. 
E. viridescens. Moderate submerged and somewhat gelatinous growth, 
suspended from large white colony occupying the whole surface of the 
liquid, depressed in the middle with wrinkled margin: conidia not observed : 
macrospores present, but relatively small. Ammonia present: red litmus 
blues rapidly. Solution alkaline. pH 8-i. Alkalinity 6 c.c. N/i HC 1 per 
litre. 
E. acremonioides. Somewhat meagre submerged growth of loose 
texture : mycelium white with terminal and intercalary swollen cells : macro¬ 
spores rare ; ends of branches resemble appressoria, probably incipient 
conidiophore formation. Ammonia not detected. Solution neutral to 
litmus. pH 6-9. Acidity equivalent to 12 c.c. N/i NaOH per litre. 
