204 
GEORGE FLEMING. 
Archives Veterinaires for November 25th, 1882 (p. 861), he 
reviews the state of the question up to the moment when he 
began his experiments; pointing out that in 1869 Grohe and 
Block produced fatal infection in rabbits, by injecting into their 
veins the spores of two of the commonest moulds, the pencillium 
glaucum and aspergillus glaucus. These results, however, were 
doubted by Cohnheim and Grawitz, who vainly attempted to 
reproduce them in 1874-75. But at a later period, in 1880, the 
last of these experimentors succeded in producing infection- with 
cultivated spores adapted to an alkaline medium. 
These experiments which Kaufmann undertook, under the 
direction of Chauveau, had reference to the aspergillus glaucus, 
and the results he obtained proved that the spores of this crypto¬ 
gam are infective without any previous adaptation. The following 
is the resume he gives of one of his experiments, those which 
were afterwards instituted in modifying the circumstances, having 
corroborated the conclusions arrived at from this one:— 
“ On May 12th, 1880, on damp bread, I sowed the spores of 
aspergillus'glaucus procured from the surface of a dried solution 
of gum arabic. This cultivation, placed in a water-bath kept at 
a temperature of 35° Cent., furnished numerous spores in about 
forty-eight hours. In order to obtain spores in abundance, I 
made a new cultivation on bread reduced to broth, with an acid 
reaction, using for this purpose the spore obtained by the preced¬ 
ing cultivation. This second crop, like the first, furnished spores 
in abundance in about forty-eight hours. I left these cultivations 
in the bath until May 19th, and on the evening of that day I put 
a quantity of spores of the second generation in water enough to 
make it look slightly turbid. Into the jugular vein of a rabbit 
(No. 1) I injected one centiliter of this fluid ; and into another 
rabbit (No. 2) two centiliters. During the night of the 23d-24th, 
rabbit No. 1 died; while rabbit No. 2 was very ill, turning its 
head towards the side and foaming at the mouth ; it died during 
the night of the 24th-25th. At the autopsy there were found in 
both rabbits the typical lesions of infection by moulds, such as 
Grawitz had described. The kidneys were highly congested in 
places, and on their surface were a multitude of white nodular 
