DU CHARBON BACTERIEN 
499 
or cultures are products of the vibrion and cause the disease .— 
Cultures of this microbe in serum, beef and veal broth were nega¬ 
tive. If tubes containing infected blood were charged with car¬ 
bonic acid, the cultures could be continued to the third genera¬ 
tion. Finally success was obtained in a mixture of chicken broth, 
glycerine and sulphate of iron, in which the bacteria can be bred 
indefinitely. 
Resistance of the microbe to causes of destruction : 
Cold. —Liquid containing virulent bacteria, frozen solid for 
forty-eight hours, retained its infecting property. 
Heat on fresh virus. —It requires a temperature of 100° C. 
(212° F.) for at least twenty minutes to destroy the virulence 
of the microbes. Boiling water poured on the virus will not , 
however, produce the same result. 
On dried virus. —When mixed with water it requires a tem¬ 
perature of 110° C. for six hours to destroy the virus; when 
placed in a tube and subjected to 110° C. it requires only two 
hours. 
Action of water or humidity. —Dropped in water the dried 
virus sinks to the bottom and may lose its infecting power in 120 
hours or may retain it for an indefinite period (exceeding three 
months). 
Putrefaction , anthrax and butyoric ferments. —The agents of 
these do not interfere with the preservation of the virus. 
Chemical agents. —These experiments were all made by 
placing the virus in contact with the substance for forty-eight 
hours and five drops of virus was then injected into a guinea pig. 
The reader will probably find the following tables useful not only 
in regard to blackleg, but also for other parasitic poisons. 
A. Action of Solutions or Liquid Substances on the Fresh Virus. 
Do not Destroy the Virulence. 
Alcohol at 90 per cent. 
Spirits camphor. 
Carbolized alcohol (saturated). 
Glycerine. 
Ammonia. 
Acetate of ammonia. 
Sulphate “ 
Sulphliydrate of ammonia. 
Carbonate * ‘ 
Do Destroy the Virulence. 
Acid carbolic (watery solution), T § 
“ salicylic, TB X TO - 
“ boric, 
“ nitric, 
“ sulphuric, (diluted). 
‘‘ muriatic, £, 
“ oxalic (saturated). 
Salicylated alcohol. 
Soda. 
