PATHOLOGICAL PHYSIOLOGY. 
517 
The cultures were made every twenty-four hours, in an oven 
heated to 38 degrees, as follows: 
The bouillon used was made of beef, heated at from 115 to 
120 degrees, in a bath of chlorure of calcium. All the precau¬ 
tions indicated for the destruction by proper heat of all the germs 
of the vases, were taken. The sowing was then made for the 
subsequent cultures, with a drop taken, with a heated tube, in a 
prepared culture, and put into a vase containing the bouillon pre¬ 
pared for another culture. 
In each vase thus sown, the liquid became cloudy after twenty- 
four hours, and the microscope revealed the fact that the cloudi¬ 
ness was due to the presence of moving organisms, of a round or 
elongated form. 
These organisms belong to the order of the aerobics, for it is 
impossible to obtain their growth in tubes free from air. 
They were easily rendered visible at the moment of using. 
To color them, the violet of methyle or other reagents, such as 
the blue of methylene, were employed. These reagents, and 
principally the last, lias made them plainly visible in the tissues 
where they existed. 
In some few of the cultures, the disposition in chapelets 
with variable grains was observed, and also ovoid organisms. 
INOCULATION OF TWO DONKEYS WITH LIQUIDS OF THE FIFTH AND 
SIXTH CULTURES. 
Donkey No. 1, with liquid of the fifth culture. —Experi¬ 
ment conducted at Alfort, on the 30th of August.—Subcutaneous 
injection made behind the left shoulder. 
Two days after, a painful swelling appears at the seat of 
inoculation, accompanied with general symptoms of extreme in¬ 
tensity; depression of the animal; extreme weakness ; trembling; 
increase of temperature, rising above 41 degrees on the last day, 
and anorexia. September 4th, the animal drops and is unable to 
rise, and dies during the night of the same day, being the sixth 
after the inoculation. Evidence of the excessive activity of the 
•/ 
inoculated virus. 
The autopsy , made September 5th, shows in the lungs, the 
typical lesions of acute glanders. These organs were literally 
