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D. E. SALMON 
3. Virulent matters containing rods only lose their activity in 
a few days if dried. 
4. Such matters containing spores retain their activity an in¬ 
definite time when dried. 
5. Virus containing rods only soon loses its activity if deprived 
of Oxygen. 
6. If these rods have formed spores, the activity is retained 
indefinitely, though deprived of oxygen. 
7. Putrefaction destroys virus which does not contain spores, 
if the access of oxygen is restricted. 
8. When there is sufficient, access of air to allow formation of 
spores, putrefaction has no effect on the virus. 
9. Virulent liquids containing rods alone lose their activity by 
being largely diluted with distilled water. 
10. The addition of water has no effect on the virulence of 
liquids containing spores. 
11. Virulent liquids, in which the bacillus has not formed 
spores, lose their activity in a few days if kept at 8° C. 
12. If spores have formed, such liquids may be kept at this 
temperature indefinitely, and retain their original activity. 
13. Virulent liquids containing rods alone lose their activity 
when treated with compressed oxygen. 
14. Such liquids in which spores have formed are not affected 
by this agent. 
15. The virulence is also destroyed by concentrated alcohol 
before spores have formed. 
16. After spore-formation this agent has no effect on the 
virulence. 
We have here a series of sixteen facts, showing the connec¬ 
tion between the activity of charbon virus and the presence of the 
living Bacillus aiithracis; these facts have been observed and con¬ 
firmed by the most accomplished investigators of the time, and I 
take it for granted they are entirely reliable. If they had all 
been announced by one man, we would be perfectly justified in 
making certain reservations before accepting them; but when we 
have the united testimony of such men as Koch, Cohn, Buchner 
and Nageli among the Germans ; Pasteur, Toussaint and Bert in 
