RESUMED STUDY OF ANTHRAX. 
191 
by scissiparity, and not by sporulation, or by the development 
of germ corpuscles, as might be supposed. * 
The duration of the inculcation is at least equal to the time 
which elapses between the introduction of the leptothrix into the 
tissues, and that when it enters the circulation. Experimental 
observation puts the maximum duration at eleven days. Ordinarily 
it is very short. By natural infection it is not possible accurately 
to define the time. 
The diagnosis of anthrax, whatever external form it may as¬ 
sume, is established with certainty by the presence of the bac- 
teridies in the organism. The media where these are found most 
abundant are the blood, the splenic pulp, the lymph of affected 
ganglious, the serosity which infiltrates the swelling, tumors and 
intestinal extravasations, and the urine. It is there that they 
must be looked for. They are found there under two conditions, 
namely, that of spores or germ-corpuscles and that of mycelium 
or batonnets. It is in the blood, the urine, and the pathological 
products that they develop themselves after the infection has 
become general. The mycrophites appear most abundant under 
the last form. They are easily detected in these two forms, with 
a magnifying power of 500 to 600. 
The free spores or microccoci have the aspect of small vesicu¬ 
lar dots, ovoid or spheroid, shying and strongly refringent. 
The spores or germs, the products of the endogenesis, appear 
in the living animal economy only after a relatively long time. 
According to M. Toussaintf the sporulation in the liquids of 
culture requires about 48 hours; a fortiori, a longer time will be 
required in the media in the economy which are less favorable to 
the growth of bacteridie. 
* Germs-corpuscles or spores find their condition better for growth and de_ 
velopment outside of the organism, for instance in the liquids of cultivation. The 
mycrophite in the state of bacteridie, that is, such as found in the circulatory 
fluids, do not grow. It contains germs-corpuscles, but these do not change into 
bacteridies into the blood. However, whether the germs come from the bac- 
teridies in the blood or directly from outside, when they are deposited into the 
tissues of an animal susceptible to anthrax, they are transformed into bacteridies 
and multiply there in the blood by fissiparity. 
Goo cit. p. 54l 
