350 
M. H. TOUS8AINT. 
searches upon tuberculosis. It was then a question of the infec¬ 
tion of eight pigs, either by the ingestion of the tuberculous lung 
of a cow, or by inoculation of the blood of a small pig, born of a 
tuberculous parent, which fed him and had died with the disease. 
At the sitting of the 28th rf June, M. Bouley presented you, 
from me, a bottle containing pieces of lung, liver, spleen, of the 
phrenic center, diaphragm, and lymphatic glands, presenting ad¬ 
vanced lesions, obtained from a five-month-old pig, after subcuta¬ 
neous injection of two cubic centimeters of the juice of muscle o^ 
a tuberculous cow, obtained by pressure. 
Since that time I have studied tuberculosis in its various modes 
of infection, and I can say, after a number of experiments made 
upon pigs, rabbits and cats, that no contagious disease possesses a 
greater virulency. 
Inoculation in the rabbit gives as positive results as anthrax. 
It is so also in all other species used for experiments. 
In tuberculosis, all the fluids of the economy, the nasal 
mucus, saliva, scrosity from the tissues, and urine, are virulent, 
and can originate the disease. As to the virus itself, of which I 
shall give you the nature later on, it resists and keeps its action 
at a temperature which kills the bacteridie of anthrax. 
If, in human subjects, tuberculosis seems to be less virulent, 
it is because it often assumes a chronic form, which may con¬ 
tinue for years, and often even end in recovery; jet it is no less 
dangerous, and physicians know that the number of recoveries 
can be counted. Contagion is also very difficult to observe, on 
account of the slow and gradual appearance of the symptoms. 
Here are experiments which demonstrate the resistance of the 
virus , and the danger of the use of the meat and other remains of 
tuberculous animals. 
First, by squeezing with a press, I extracted from the lung of 
a tuberculous cow, having an oedema of the anterior lobe, a cer¬ 
tain quantity of fluid, slightly loaded with virus, almost trans¬ 
parent. One e.c.5 of this was injected under the skin of the in¬ 
ferior part of the ear of a young pig and ten drops to two rabbits. 
I then injected the same quantities of the same fluid, heated in a 
water bath to 55°-58° for ten minutes, to four pigs and four rab¬ 
bits in the same regions. 
