414 
M. T0TJ8SAHSTT. 
in the drying stove. After several days most of these liquids 
offered very small granulations, simple, germinated, or gathered 
in small masses. I made second cultures, and afterwards inocu¬ 
lated to young kittens; these animals live with difficulty in cap¬ 
tivity, and all died from exhaustion, before it was possible to 
observe tuberculosis Five months after having collected the 
serum, 1 had occasion to inoculate two other cats, almost adult, 
with the contents of a syringe of Pravaz, with the serum, which 
had been kept for several weeks in the drying stove, and which 
presented spherical granulations. The two cats were killed 47 
days after inoculation. 
“ One of them showed a local lesion, quite well marked, and 
a voluminous prescapular ganglion ; but the lung contained no 
tubercles. The second presented the same local or ganglionic 
lesions, and besides, some twenty very small tubercles, here and 
there, in the lungs. The microscopic examination showed that 
it was true tuberculosis. I only mention this fact to show the 
duration of the preservation of the tuberculous virus. This ex¬ 
periment is evidently insufficient to prove the existence of the 
microbe. 
“ The 1st of March I killed a young sow, which had eaten four 
months before, in two days, the lung of a cow, which weighed 39 
kilog. (about 78 pounds), which came from a slaughter-house. 
The sow had well-developed tuberculosis. * * * I saved, 
with all the care necessary in such cases, some of the blood and 
pulp of the pharyngeal, pulmonary and intestinal ganglions, and 
I made a culture in seven bottles, containing slightly alkaline 
bouillon of rabbit. As early as the next day they were cloudy, 
and all contained one and the same microbe. These cultures, 
carried to the 10th, all preserved their purity. The activity of 
the growth lasts from 10 to 15 days, after which the fluid clears 
off, and the microbe fall to the bottom of the bottle and form a 
deposit slightly yellow in color. Their refringency is then 
greater than at the beginning of the experiment, and their 
diameter has diminished, becoming a little below that of the 
microbe of chicken cholera. 
“ The first inoculations of these cultures were made on rabbits, 
