246 
GEORGE FLEMING. 
They were all inoculated with small-pox matter, between Decem¬ 
ber and April, the material being obtained from four people 
who were affected with small-pox, and who had never been 
vaccinated; it was'always employed very fresh. The inocula¬ 
tions were made at the vulva in the females, and perineum 
and scrotum in the males, and exactly in the same way as in 
vaccinating—the punctures being sometimes subepidermic, at , 
other times quite subcutaneous. In none of the animals were 
there observed the slightest general phenomena—no disseminated 
eruption, fever, loss of appetite, or diminution of the lacteal 
secretion. With regard to the local phenomena, they were so 
trifling that in a first series of experiments they were inappreci¬ 
able ; hence the error of some of the .preceding experimenters, 
who conclude that cattle could not be successfully inoculated with 
Small-pox virus. But other experiments prove this to be a 
fallacy; and a drawing accompanying the report shows the 
perineal region of a bull-calf, and on the left side of which five 
sub-epidermic variolous inoculations had been made, and the 
effect of which had arrived at its summum of development. 
There were small, red, slightly-prominent papules, from two to 
four millimeters in diameter, and slightly conical, in the centre of 
which the inoculation-puncture could be distinguished. These 
papules commenced to develop on the second day, and on the 
fifth had arrived at the dimensions shown in the drawing. On 
the twelfth day they had completely disappeared, after furnishing 
at the seat of puncture, an extrqpiely small, darkish crust. The 
course was not observed, however, in all the twelve inoculated 
animals; but it was remarked that the deep punctures did not 
furnish more evident results that the subepidermic ones. The 
former, though made with a canulated' needle highly charged 
with virus, only gave rise, indeed, to an eruption in which the 
papules were not so red or circumscribed as in the latter, and fre¬ 
quently could only be distinguished in the skin by passing the 
finger over them. Some deep inoculations were made by pass¬ 
ing the virus through a wound several millimeters in extent, made 
by a lancet. The results were no more marked. 
(To be continued .) 
