266 
M. H. TOUSSATNT. 
were brothers. The four vaccinated animals resisted four suc¬ 
cessive inoculations by injection of carbuncular blood, made under 
the skin. The four others not vaccinated died by the first inocu¬ 
lation, within from two to four days, with large oedema at the 
point of inoculation. The nearest ganglion had increased from 
ten to fifteen times beyond its natural size. It was filled with 
bacteridies; their number was much higher than that of the blood 
corpuscles. 
At the first inoculation of anthrax, the vaccinated animals 
had a little fever, and in two there was a very slight oedema at 
the point of inoculation The other points of inoculation acted 
as simple wounds. 
Sheep.— They belonged to the breed of Lauraguais, in which 
anthrax called spontaneous is very common and fatal. My ex¬ 
periments were on eleven animals. Five were inoculated once 
but at different times, and died of it in two or three days. I 
have never seen one of this breed, which I employed for some 
years in my experiments, to resist the injection of bacteridies, 
whatever quantity was used. 
The six remaining were inoculated with anthrax, and one died 
with the ordinary symptoms. The five remaining received a 
second vaccination, and after the lapse of a month I made in 
each three sub-cutaneous inoculations with carbunculous blood 
of a dog, a rabbit and a ewe, and one of spores, without giving 
rise to either local or general phenomena. 
The absence of the local phenomena indicated that the blood 
itself was unfit for the reproduction of bacteridies. I have, indeed, 
introduced into the facial vein of four of these animals two or 
three drops of blood from a rabbit, which, considering the num¬ 
ber of parasites, represented for each animal a total of about 
two hundred millions of bacteridies introduced directly in the 
blood. Those four sheep presented no morbid phenomena. 
To-day these animals are in good health and are not at all 
affected by the different processes of vaccination or inoculation. 
They will return to their flock, and I propose to inoculate them 
from time to time to find out the length of this innocuity. I 
can now affirm that it will last more than two months. 
