OSTHO SARCOMA-SPINA VENTOSA. 
267 
Mode of Inoculation.—I first used the filtration of carbunc- 
ular blood from a dog, sheep or rabbit. For that purpose I took 
the blood of an inoculated animal, as lie was about dying or 
immediately after death. This blood was defibrinated by beating, 
run through a cloth and filtrated through ten or twelve sheets of 
paper. It is by this process that three pups three months old, 
and the first ewe, were vaccinated. But this is a dangerous method 
and not at all practical, as often the fissures allow bacteridies to 
pass through, and they cannot be recognized with the microscope, 
as they are very few, and then animals which we wished to save 
were killed. 
Being unable to obtain a filter giving a sufficient quantity of 
filtrated matter, I used heat to kill the bacteridies, and exposed 
the defibrinated blood to 55° temperature for ten minutes. The' 
result was complete. Five sheep vaccinated with three cubic 
centimeters of this blood have been inoculated with very active 
carbunculous blood and have shown no effect from it. 
However, to obtain a complete immunity, it is necessary to 
make several inoculations. Thus, after the first preventative in¬ 
oculations, I inserted under the skin of the ear of two sheep car¬ 
bunculous blood of the rabbit and spores of culture. One of 
these died with a large number of bacteridies in the blood. 
I then inoculated again the four remaining sheep with the 
blood of the dead sheep, after heating it to 55°, and since that 
time each sheep has been inoculated twice with carbunculous 
blood without result. 
OSTEO SARCOMA—SPINA VENTOSA, 
(Read before the United States Veterinary Medical Association.) 
In speaking of diseased processes of bones, Zundel, in his 
excellent Review of D’Arboval’s Dictionary, says: “ Sarcoma 
have particularly been observed in the bones of the face, where 
they may assume considerable size; the bones which are thus 
affected are then tumefied, puffed up, as in spina ventosa. They 
