GEORGE FLEMING. 
2*0 
eacli arm, all of which had a bouton ; in eight days from inocula¬ 
tion the development of these was complete, and they so closely 
resembled vaccine pustules that several competent persons as¬ 
serted they were such. The next day two small vesicles appeared 
around each pustule, and in four days some small-pox pustules 
(about fifteen) showed themselves on the face and other parts of 
the body. The child did not appear to be unwell, and the slight 
general eruption disappeared rapidly withont leaving any traces. 
Thus, in a second generation in the human species the variolic 
virus from the cow had produced much better local effects than 
in the first, and incomparably feebler general effects. What might 
it have been in a third or a fourth generation ? The fear of 
creating an outbreak of small-pox in the hospital brought these 
transmissions to a conclusion. But even the very decided results 
obtained did not suffice to satisfy the Commission. After all, the 
eruption on the children, though it appeared to be due to nothing 
more than direct inoculation of human small-pox, might be only 
generalized vaccinia, which some authorities assert does occur. 
But such a confusion between the two diseases was proved by the 
foregoing experiments to be impossible. Deposit humanized vac¬ 
cine beneath the skin of a calf, and an eruption of unmistakable 
vaccinal pustules is obtained, which can be indefinitely transmit¬ 
ted to animals of the same species. But inoculate with small-pox 
virus, and there is only an insignificant papular eruption, the 
transmission of which from one calf to another is extremely 
difficult, if not impossible. 
To meet any objections, however, that might afterwards be 
raised in this respect, two calves—a male and a female—were in¬ 
oculated with virus from the last-mentioned child. In seven days, 
in both animals, there was a somewhat microscopic papule at the 
seat of puncture. One of these papules—on the scrotum of the 
male—was used for the inoculation of another calf, but the re¬ 
sult was absolutely negative ; proving, in the first place, that the 
virus transferred from small-pox infected man to bovines, and 
carried back to man again, is still small pox virus, and is not con¬ 
verted into cow-pox virus ; and in the second place, that small-pox 
is not vaccinia. 
