EXPERIMENTAL ANALYSIS OF MALIGNANT PUSTULE, ETC. 363 
where it has been deposited, so much so, that carried upon a rab¬ 
bit, it kills him with its ordinary rapidity. After twenty-four 
hours, when the carbunculous pustule is well defined, the serous 
liquid which escapes spontaneously from the puncture, or which 
is squeezed by pressure, is apt to give anthrax to the rabbit, 
guinea pig, and to all the animals liable to contract it. That 
which is obtained the second, third or fourth day, still possesses its 
activity. Here are the proofs. 
The serosity of a carbunculous oedema of the dog in the 
twentieth hour killed a rabbit in four days. 
That of a malignant pustule in an animal of the same species 
proved virulent at the twenty-fourth hour. That of the oedema 
of another, virulent at the forty-eighth hour. 
That of a carbunculous tumor was virulent at the forty-eighth 
hour and killed at the seventieth. 
That of a malignant pustule of forty-eight hours killed a rat. 
The serosity of a tumor of seventy-two hours has also proved 
virulent. 
That taken from the interior of a malignant pustule, which 
had proved fatal after six days, had yet a weak virulency. 
On the contrary, that property was often absent in the same 
liquids, though they contained bacterides, even at an epoch very 
near the first moments of the evolution of the tumors, pustules 
or oedema. 
For instance, the serosity of the oedema of a cat became 
sterile at the forty-second hour. 
That of a pustule of fifty hours produced no effect. 
The bloody serosity obtained on the dog by scarifications on a 
tumor of one hundred and twenty hours, produced no accident. 
The serosity oozing from a malignant pustule of a dog at the 
seventy-third hour, was sterile. 
The serosity of the phlyctence of the same pustule at the sev¬ 
enty-fourth hour, sterile. In those three liquids there were some 
bacterides and many shying granules, having the character of the 
corpuscle germs of bacterides. 
Finally, the serosity of a carbunculous oedema of the horse, 
and the reddish liquid of a carbunculous tumor of the same 
