PLEURO-PNEUMONIA. 
413 
stroying the life of the animal; finally, purulent affection (septicaemia) 
has occasionally followed with even more fatal results. As I shall at¬ 
tempt to show, however, these complicated and fatal results have gen¬ 
erally resulted from the use of improper inoculating- material ; from 
improper methods of inoculation, or from improper care of the animals. 
• \ t P 
WHERE AND HOW TO INOCULATE. 
Inoculations have been made in various parts of the body, particu¬ 
larly in the dewlap, nose, ear, root and tip of tail ; but, from the many 
complications which I have just mentioned as following the operation, 
the tip of the tail is now universally accepted as the safest pdint. If 
unhealthy tumors form, a small part of the organ can be amputated 
without trouble or danger ; if dry gangrene results, the loss of a few 
inches of the tail is of little consequence ; and being further from the 
vital organs, the inflammation, gangrene, &c., is less apt to extend to 
them. 
The greatest care must be exercised in selecting and preserving 
the virus. Formerly it was very common to use the purulent discharge 
from the tail, or the liquids from the lungs in the last stages of the 
disease, or even the serum from gangrenous lungs, while, even at the 
present time, dairymen inoculate their own animals by binding pieces 
of the lung on wounds made in the tail, and still others go so far as to 
preserve such virus, and use it after it has decomposed and become 
fetid. It has been shown, again and again, that serious complications 
and a high death rate invariably follow such careless methods. The 
French commission, which inoculated with the liquids taken from the 
lungs of animals in the later stages of the disease, lost over eleven 
per cent, of the animals ; while, when the liquid is obtained in the 
earlier stages of the disease, and properly preserved, the loss should be 
less than one per cent. When purulent matter is used, or that which 
has decomposed by keeping, or where slices of lung are kept applied, 
purulent infection is a very common result. It is, then, a matter of 
the utmost importance to use proper virus, and I shall, therefore, de¬ 
scribe in detail the methods of obtaining and using it. 
M. Boulay, of Aresnes, France, and his colleagues in the same . 
district, have probably been the most successful of any who have prac¬ 
ticed inoculation ; of 728 animals inoculated, not one died ! He thus 
describes the manner in which the virus was obtained and the animals 
inoculated : 
“ Such portions of the lung as show red hepatization are pressed 
